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Immunology Adaptive Immunity T Cells Non-CD
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RecombinantRabMAb

Recombinant Anti-LSP1 antibody [EPR3160] (ab134099)

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Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
  • Anti-LSP1 antibody [EPR3160] (ab134099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3160] to LSP1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-LSP1 antibody [EPR3160]
    See all LSP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3160] to LSP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LSP1 aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Daudi, Ramos, HuT-78, U937 and K562 cell lysates; Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3160
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Alternative Versions

    • Anti-LSP1 antibody [EPR3160] - BSA and Azide free (ab248682)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Daudi whole cell lysate (ab3951)
    • Ramos whole cell lysate (ab3955)

Applications

Our Abpromise guarantee covers the use of ab134099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      May play a role in mediating neutrophil activation and chemotaxis.
    • Tissue specificity

      Activated T-lymphocytes.
    • Post-translational
      modifications

      Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P33241 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4046 Human
      • Omim: 153432 Human
      • SwissProt: P33241 Human
      • Unigene: 56729 Human
      • Alternative names

        • 47 kDa actin binding protein antibody
        • 47 kDa actin-binding protein antibody
        • 52 kDa phosphoprotein antibody
        • F actin binding and cytoskeleton associated protein antibody
        • Leufactin (leukocyte F-actin binding protein) antibody
        • Leufactin antibody
        • Leukocyte F actin binding protein antibody
        • Leukocyte specific protein 1 antibody
        • LSP 1 antibody
        • Lsp1 antibody
        • LSP1_HUMAN antibody
        • Lymphocyte specific antigen WP34 antibody
        • Lymphocyte specific protein 1 antibody
        • Lymphocyte specific protein pp52 antibody
        • Lymphocyte-specific antigen WP34 antibody
        • Lymphocyte-specific protein 1 antibody
        • OTTHUMP00000014138 antibody
        • OTTHUMP00000014139 antibody
        • OTTHUMP00000014140 antibody
        • OTTHUMP00000069612 antibody
        • OTTHUMP00000069614 antibody
        • OTTHUMP00000069615 antibody
        • OTTHUMP00000069616 antibody
        • pp52 antibody
        • Protein pp52 antibody
        • S37 protein antibody
        • WP 34 antibody
        • WP34 antibody
        see all

      Images

      • Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
        Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
        All lanes : Anti-LSP1 antibody [EPR3160] (ab134099) at 1/1000 dilution

        Lane 1 : Daudi cell lysate
        Lane 2 : Ramos cell lysate
        Lane 3 : HuT-7 cell lysate
        Lane 4 : U937 cell lysate
        Lane 5 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab134099 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human colon lymphocytes tissue using ab134099 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human breast lymphocytes tissue using ab134099 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134099 showing -ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134099 showing -ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab134099 showing -ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-LSP1 antibody [EPR3160] (ab134099)
        Anti-LSP1 antibody [EPR3160] (ab134099)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab134099? Please let us know so that we can cite the reference in this datasheet.

      ab134099 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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