Recombinant
RabMAb

Recombinant Anti-LSP1 antibody [EPR3160] (ab134099)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3160] to LSP1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LSP1 antibody [EPR3160]
    See all LSP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3160] to LSP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human LSP1 aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Daudi, Ramos, HuT-78, U937 and K562 cell lysates; Human tonsil tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab134099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      May play a role in mediating neutrophil activation and chemotaxis.

    • Tissue specificity

      Activated T-lymphocytes.

    • Post-translational
      modifications

      Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 47 kDa actin binding protein antibody
        • 47 kDa actin-binding protein antibody
        • 52 kDa phosphoprotein antibody
        • F actin binding and cytoskeleton associated protein antibody
        • Leufactin (leukocyte F-actin binding protein) antibody
        • Leufactin antibody
        • Leukocyte F actin binding protein antibody
        • Leukocyte specific protein 1 antibody
        • LSP 1 antibody
        • Lsp1 antibody
        • LSP1_HUMAN antibody
        • Lymphocyte specific antigen WP34 antibody
        • Lymphocyte specific protein 1 antibody
        • Lymphocyte specific protein pp52 antibody
        • Lymphocyte-specific antigen WP34 antibody
        • Lymphocyte-specific protein 1 antibody
        • OTTHUMP00000014138 antibody
        • OTTHUMP00000014139 antibody
        • OTTHUMP00000014140 antibody
        • OTTHUMP00000069612 antibody
        • OTTHUMP00000069614 antibody
        • OTTHUMP00000069615 antibody
        • OTTHUMP00000069616 antibody
        • pp52 antibody
        • Protein pp52 antibody
        • S37 protein antibody
        • WP 34 antibody
        • WP34 antibody
        see all

      Images

      • Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
        Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
        All lanes : Anti-LSP1 antibody [EPR3160] (ab134099) at 1/1000 dilution

        Lane 1 : Daudi cell lysate
        Lane 2 : Ramos cell lysate
        Lane 3 : HuT-7 cell lysate
        Lane 4 : U937 cell lysate
        Lane 5 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab134099 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human colon lymphocytes tissue using ab134099 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human breast lymphocytes tissue using ab134099 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134099 showing -ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134099 showing -ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)

        Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab134099 showing -ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      Publishing research using ab134099? Please let us know so that we can cite the reference in this datasheet.

      ab134099 has not yet been referenced specifically in any publications.

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