Recombinant Anti-LSP1 antibody [EPR3160] (ab134099)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3160] to LSP1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-LSP1 antibody [EPR3160]
See all LSP1 primary antibodies -
Description
Rabbit monoclonal [EPR3160] to LSP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LSP1 aa 50-150 (internal sequence). The exact sequence is proprietary.
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Positive control
- Daudi, Ramos, HuT-78, U937 and K562 cell lysates; Human tonsil tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3160 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab134099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 37 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in mediating neutrophil activation and chemotaxis. -
Tissue specificity
Activated T-lymphocytes. -
Post-translational
modificationsPhosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4046 Human
- Omim: 153432 Human
- SwissProt: P33241 Human
- Unigene: 56729 Human
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Alternative names
- 47 kDa actin binding protein antibody
- 47 kDa actin-binding protein antibody
- 52 kDa phosphoprotein antibody
see all
Images
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All lanes : Anti-LSP1 antibody [EPR3160] (ab134099) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HuT-7 cell lysate
Lane 4 : U937 cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab134099 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
Immunohistochemical analysis of paraffin embedded normal Human colon lymphocytes tissue using ab134099 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
Immunohistochemical analysis of paraffin embedded normal Human breast lymphocytes tissue using ab134099 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134099 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134099 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab134099 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab134099 has not yet been referenced specifically in any publications.