Recombinant
RabMAb

Recombinant Anti-LSP1 antibody [EPR3160] (ab134099)

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Overview

  • Product name

    Anti-LSP1 antibody [EPR3160]
    See all LSP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3160] to LSP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human LSP1 aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Daudi, Ramos, HuT-78, U937 and K562 cell lysates; Human tonsil tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      May play a role in mediating neutrophil activation and chemotaxis.

    • Tissue specificity

      Activated T-lymphocytes.

    • Post-translational
      modifications

      Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 47 kDa actin binding protein antibody
        • 47 kDa actin-binding protein antibody
        • 52 kDa phosphoprotein antibody
        • F actin binding and cytoskeleton associated protein antibody
        • Leufactin (leukocyte F-actin binding protein) antibody
        • Leufactin antibody
        • Leukocyte F actin binding protein antibody
        • Leukocyte specific protein 1 antibody
        • LSP 1 antibody
        • Lsp1 antibody
        • LSP1_HUMAN antibody
        • Lymphocyte specific antigen WP34 antibody
        • Lymphocyte specific protein 1 antibody
        • Lymphocyte specific protein pp52 antibody
        • Lymphocyte-specific antigen WP34 antibody
        • Lymphocyte-specific protein 1 antibody
        • OTTHUMP00000014138 antibody
        • OTTHUMP00000014139 antibody
        • OTTHUMP00000014140 antibody
        • OTTHUMP00000069612 antibody
        • OTTHUMP00000069614 antibody
        • OTTHUMP00000069615 antibody
        • OTTHUMP00000069616 antibody
        • pp52 antibody
        • Protein pp52 antibody
        • S37 protein antibody
        • WP 34 antibody
        • WP34 antibody
        see all

      Images

      • Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
        Western blot - Anti-LSP1 antibody [EPR3160] (ab134099)
        All lanes : Anti-LSP1 antibody [EPR3160] (ab134099) at 1/1000 dilution

        Lane 1 : Daudi cell lysate
        Lane 2 : Ramos cell lysate
        Lane 3 : HuT-7 cell lysate
        Lane 4 : U937 cell lysate
        Lane 5 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab134099 at 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemical analysis of paraffin embedded normal Human colon lymphocytes tissue using ab134099 showing +ve staining.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemical analysis of paraffin embedded normal Human breast lymphocytes tissue using ab134099 showing +ve staining.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134099 showing -ve staining.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134099 showing -ve staining.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (ab134099)
        Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab134099 showing -ve staining.

      References

      ab134099 has not yet been referenced specifically in any publications.

      Publishing research using ab134099? Please let us know so that we can cite the reference in this datasheet.

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