Recombinant Anti-LSP1 antibody [EPR5997] (ab133506)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5997] to LSP1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LSP1 antibody [EPR5997]
See all LSP1 primary antibodies -
Description
Rabbit monoclonal [EPR5997] to LSP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LSP1 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Daudi, Ramos and HuT78 cell lysates. Mouse spleen and Rat brain tissue lysates.IHC-P: Human colon, Mouse spleen, Rat spleen and Human tonsil tissue. ICC/IF: Daudi Flow Cyt: Daudi
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.02 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5997 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab133506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa). | |
IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
Flow Cyt | 1/100. | |
ICC/IF | 1/100. For unpurified use at 1/500 - 1/1000. |
Target
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Function
May play a role in mediating neutrophil activation and chemotaxis. -
Tissue specificity
Activated T-lymphocytes. -
Post-translational
modificationsPhosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4046 Human
- Entrez Gene: 16985 Mouse
- Entrez Gene: 361680 Rat
- Omim: 153432 Human
- SwissProt: P33241 Human
- SwissProt: P19973 Mouse
- Unigene: 56729 Human
- Unigene: 234003 Mouse
see all -
Alternative names
- 47 kDa actin binding protein antibody
- 47 kDa actin-binding protein antibody
- 52 kDa phosphoprotein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified) + Rat brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa -
All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified)
Lane 1 : Mouse spleen lysates
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HuT78 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (2)
ab133506 has been referenced in 2 publications.
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Zhang H et al. Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation. FEBS Open Bio 6:1227-1237 (2016). PubMed: 28255535