Recombinant
RabMAb

Recombinant Anti-LSP1 antibody [EPR5997] (ab133506)

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Overview

  • Product name

    Anti-LSP1 antibody [EPR5997]
    See all LSP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5997] to LSP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human LSP1 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Daudi, Ramos and HuT78 cell lysates. Mouse spleen and Rat brain tissue lysates. IHC-P: Human colon, Mouse spleen, Rat spleen and Human tonsil tissue. ICC/IF: Daudi FC: Daudi

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Flow Cyt 1/100.
ICC/IF 1/100.

For unpurified use at 1/500 - 1/1000.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      May play a role in mediating neutrophil activation and chemotaxis.

    • Tissue specificity

      Activated T-lymphocytes.

    • Post-translational
      modifications

      Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 47 kDa actin binding protein antibody
      • 47 kDa actin-binding protein antibody
      • 52 kDa phosphoprotein antibody
      • F actin binding and cytoskeleton associated protein antibody
      • Leufactin (leukocyte F-actin binding protein) antibody
      • Leufactin antibody
      • Leukocyte F actin binding protein antibody
      • Leukocyte specific protein 1 antibody
      • LSP 1 antibody
      • Lsp1 antibody
      • LSP1_HUMAN antibody
      • Lymphocyte specific antigen WP34 antibody
      • Lymphocyte specific protein 1 antibody
      • Lymphocyte specific protein pp52 antibody
      • Lymphocyte-specific antigen WP34 antibody
      • Lymphocyte-specific protein 1 antibody
      • OTTHUMP00000014138 antibody
      • OTTHUMP00000014139 antibody
      • OTTHUMP00000014140 antibody
      • OTTHUMP00000069612 antibody
      • OTTHUMP00000069614 antibody
      • OTTHUMP00000069615 antibody
      • OTTHUMP00000069616 antibody
      • pp52 antibody
      • Protein pp52 antibody
      • S37 protein antibody
      • WP 34 antibody
      • WP34 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
      Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
      Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified) + Rat brain lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 37 kDa

    • Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
      Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
      All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified)

      Lane 1 : Mouse spleen lysates
      Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
      Lane 3 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 37 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunocytochemistry/ Immunofluorescence - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry - Anti-LSP1 antibody [EPR5997] (ab133506)
      Flow Cytometry - Anti-LSP1 antibody [EPR5997] (ab133506)
      Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
      Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
      All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : HuT78 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 37 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Other - Anti-LSP1 antibody [EPR5997] (ab133506)
      Other - Anti-LSP1 antibody [EPR5997] (ab133506)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Chen X  et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). Read more (PubMed: 31293967) »
    • Zhang H  et al. Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation. FEBS Open Bio 6:1227-1237 (2016). Read more (PubMed: 28255535) »
    See all 2 Publications for this product

    Publishing research using ab133506? Please let us know so that we can cite the reference in this datasheet.

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