Recombinant Anti-LSP1 antibody [EPR5997] (ab133506)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5997] to LSP1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-LSP1 antibody [EPR5997]
See all LSP1 primary antibodies
Description

Rabbit monoclonal [EPR5997] to LSP1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human LSP1 aa 300-400 (C terminal). The exact sequence is proprietary.
Positive control
- WB: Daudi, Ramos and HuT78 cell lysates. Mouse spleen and Rat brain tissue lysates.IHC-P: Human colon, Mouse spleen, Rat spleen and Human tonsil tissue. ICC/IF: Daudi Flow Cyt (intra): Daudi
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.02 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5997
Isotype

IgG
Research areas

Alternative Versions
- Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Daudi whole cell lysate (ab3951)
- Ramos whole cell lysate (ab3955)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF		1/100. For unpurified use at 1/500 - 1/1000.
Flow Cyt (Intra)		1/100.

Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/100. For unpurified use at 1/500 - 1/1000.
Flow Cyt (Intra) 1/100.

Application notes

Is unsuitable for IP.

Function

May play a role in mediating neutrophil activation and chemotaxis.
Tissue specificity

Activated T-lymphocytes.
Post-translational
modifications

Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P33241 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4046 Human
- Entrez Gene: 16985 Mouse
- Entrez Gene: 361680 Rat
- Omim: 153432 Human
- SwissProt: P33241 Human
- SwissProt: P19973 Mouse
- Unigene: 56729 Human
- Unigene: 234003 Mouse
Alternative names
- 47 kDa actin binding protein antibody
- 47 kDa actin-binding protein antibody
- 52 kDa phosphoprotein antibody
- F actin binding and cytoskeleton associated protein antibody
- Leufactin (leukocyte F-actin binding protein) antibody
- Leufactin antibody
- Leukocyte F actin binding protein antibody
- Leukocyte specific protein 1 antibody
- LSP 1 antibody
- Lsp1 antibody
- LSP1_HUMAN antibody
- Lymphocyte specific antigen WP34 antibody
- Lymphocyte specific protein 1 antibody
- Lymphocyte specific protein pp52 antibody
- Lymphocyte-specific antigen WP34 antibody
- Lymphocyte-specific protein 1 antibody
- OTTHUMP00000014138 antibody
- OTTHUMP00000014139 antibody
- OTTHUMP00000014140 antibody
- OTTHUMP00000069612 antibody
- OTTHUMP00000069614 antibody
- OTTHUMP00000069615 antibody
- OTTHUMP00000069616 antibody
- pp52 antibody
- Protein pp52 antibody
- S37 protein antibody
- WP 34 antibody
- WP34 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)

Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified) + Rat brain lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 37 kDa
Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)

All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified)

Lane 1 : Mouse spleen lysates
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-LSP1 antibody [EPR5997] (ab133506)

Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-LSP1 antibody [EPR5997] (ab133506)

Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)

All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution

Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HuT78 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
OI-RD Scanning - Anti-LSP1 antibody [EPR5997] (ab133506)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-LSP1 antibody [EPR5997] (ab133506)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab133506? Please let us know so that we can cite the reference in this datasheet.

ab133506 has been referenced in 2 publications.

Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
Zhang H et al. Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation. FEBS Open Bio 6:1227-1237 (2016). PubMed: 28255535

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (2)

Customer reviews and Q&As

Dissociation constant (K_D)

Post-translational
modifications