Anti-LT subunit (A + B) antibody (ab188541)
Key features and details
- Rabbit polyclonal to LT subunit (A + B)
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-LT subunit (A + B) antibody -
Description
Rabbit polyclonal to LT subunit (A + B) -
Host species
Rabbit -
Specificity
ab188541 reacts with LT and cholera toxin. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Escherichia coli LT subunit (A + B). Initial immunization with LT toxoid and booster with LT toxin.
Database link: P06717 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab188541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 30 kDa. | |
| IP | Use at an assay dependent concentration. |
Images
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Western blot - Anti-LT subunit (A + B) antibody (ab188541)Lane 1 : Anti-LT subunit (A + B) antibody (ab188541)
Lane 2 : Anti-E. coli heat labile toxin A antibody [EC01] (ab243105)
All lanes : Culture medium of E. coli(ETEC, LT+)
Predicted band size: 30 kDa -
Immunoprecipitation - Anti-LT subunit (A + B) antibody (ab188541)Immunopprecipitation of LT from culture medium of ETEC by using ab188541. H chain and L chain indicate heavy chain and light chain of IgG, respectively.
Protocols
Datasheets and documents
References (2)
ab188541 has been referenced in 2 publications.
- Lu X et al. Heat-Labile Enterotoxin-Induced PERK-CHOP Pathway Activation Causes Intestinal Epithelial Cell Apoptosis. Front Cell Infect Microbiol 7:244 (2017). WB ; Human . PubMed: 28642847
- Lu X et al. Electron Acceptors Induce Secretion of Enterotoxigenic Escherichia coli Heat-Labile Enterotoxin under Anaerobic Conditions through Promotion of GspD Assembly. Infect Immun 84:2748-57 (2016). PubMed: 27430271
