Anti-LT subunit (A + B) antibody (ab188541)
Key features and details
- Rabbit polyclonal to LT subunit (A + B)
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-LT subunit (A + B) antibody -
Description
Rabbit polyclonal to LT subunit (A + B) -
Host species
Rabbit -
Specificity
ab188541 reacts with LT and cholera toxin. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Escherichia coli LT subunit (A + B). Initial immunization with LT toxoid and booster with LT toxin.
Database link: P06717 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
|
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
|
IP
Use at an assay dependent concentration. |
Images
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Western blot - Anti-LT subunit (A + B) antibody (ab188541)Lane 1 : Anti-LT subunit (A + B) antibody (ab188541)
Lane 2 : Anti-E. coli heat labile toxin A antibody [EC01] (ab243105)
All lanes : Culture medium of E. coli(ETEC, LT+)
Predicted band size: 30 kDa -
Immunoprecipitation - Anti-LT subunit (A + B) antibody (ab188541)Immunopprecipitation of LT from culture medium of ETEC by using ab188541. H chain and L chain indicate heavy chain and light chain of IgG, respectively.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab188541 has been referenced in 3 publications.
- Dierick M et al. Porcine and Bovine Forms of Lactoferrin Inhibit Growth of Porcine Enterotoxigenic Escherichia coli and Degrade Its Virulence Factors. Appl Environ Microbiol 86:N/A (2020). PubMed: 32631861
- Lu X et al. Heat-Labile Enterotoxin-Induced PERK-CHOP Pathway Activation Causes Intestinal Epithelial Cell Apoptosis. Front Cell Infect Microbiol 7:244 (2017). WB ; Human . PubMed: 28642847
- Lu X et al. Electron Acceptors Induce Secretion of Enterotoxigenic Escherichia coli Heat-Labile Enterotoxin under Anaerobic Conditions through Promotion of GspD Assembly. Infect Immun 84:2748-57 (2016). PubMed: 27430271
