Anti-LT subunit (A + B) antibody (ab188541)
Key features and details
- Rabbit polyclonal to LT subunit (A + B)
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
-
Product name
Anti-LT subunit (A + B) antibody -
Description
Rabbit polyclonal to LT subunit (A + B) -
Host species
Rabbit -
Specificity
ab188541 reacts with LT and cholera toxin. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length native protein (purified) corresponding to Escherichia coli LT subunit (A + B). Initial immunization with LT toxoid and booster with LT toxin.
Database link: P06717 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
IP
Use at an assay dependent concentration. |
Images
-
Lane 1 : Anti-LT subunit (A + B) antibody (ab188541)
Lane 2 : Anti-E. coli heat labile toxin A antibody [EC01] (ab243105)
All lanes : Culture medium of E. coli(ETEC, LT+)
Predicted band size: 30 kDa -
Immunopprecipitation of LT from culture medium of ETEC by using ab188541. H chain and L chain indicate heavy chain and light chain of IgG, respectively.
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab188541 has been referenced in 4 publications.
- Berzosa M et al. Immune Response after Skin Delivery of a Recombinant Heat-Labile Enterotoxin B Subunit of Enterotoxigenic Escherichia coli in Mice. Pharmaceutics 14:N/A (2022). PubMed: 35213971
- Dierick M et al. Porcine and Bovine Forms of Lactoferrin Inhibit Growth of Porcine Enterotoxigenic Escherichia coli and Degrade Its Virulence Factors. Appl Environ Microbiol 86:N/A (2020). PubMed: 32631861
- Lu X et al. Heat-Labile Enterotoxin-Induced PERK-CHOP Pathway Activation Causes Intestinal Epithelial Cell Apoptosis. Front Cell Infect Microbiol 7:244 (2017). WB ; Human . PubMed: 28642847
- Lu X et al. Electron Acceptors Induce Secretion of Enterotoxigenic Escherichia coli Heat-Labile Enterotoxin under Anaerobic Conditions through Promotion of GspD Assembly. Infect Immun 84:2748-57 (2016). PubMed: 27430271