Anti-LTA antibody (ab227929)
Key features and details
- Rabbit polyclonal to TNF beta
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LTA antibody
See all TNF beta primary antibodies -
Description
Rabbit polyclonal to TNF beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment within Human LTA (internal sequence). The exact sequence is proprietary.
Database link: P01374 -
Positive control
- WB: Rat and human spleen tissue extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
Target
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Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. -
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated. - Information by UniProt
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Database links
- Entrez Gene: 494185 Chimpanzee
- Entrez Gene: 4049 Human
- Entrez Gene: 25008 Rat
- Entrez Gene: 715425 Rhesus monkey
- Omim: 153440 Human
- SwissProt: P61125 Chimpanzee
- SwissProt: P01374 Human
- SwissProt: Q06332 Rat
see all -
Alternative names
- LTalpha antibody
- DAMA-25N12.13-004 antibody
- DIF antibody
see all
Images
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Western blot - Anti-LTA antibody (ab227929)Anti-LTA antibody (ab227929) at 1/500 dilution + Rat spleen tissue extract at 30 µg
Predicted band size: 22 kDa12 % SDS-PAGE gel.
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Western blot - Anti-LTA antibody (ab227929)Anti-LTA antibody (ab227929) at 1/500 dilution + Human spleen tissue extract at 30 µg
Predicted band size: 22 kDa12% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227929 has been referenced in 1 publication.
- Zhou L et al. Human Bone Mesenchymal Stem Cell-Derived Exosomes Inhibit IL-1ß-Induced Inflammation in Osteoarthritis Chondrocytes. Cell J 23:485-494 (2021). PubMed: 34455725