Overview

  • Product name

  • Description

    Rabbit polyclonal to LTB
  • Host species

    Rabbit
  • Specificity

    ab64835 detects endogenous levels of total Lymphotoxin Beta protein.
  • Tested applications

    Suitable for: IHC-P, ELISA, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human LTB (C terminal).

  • Positive control

    • HepG2 cell extracts and HeLa cells.
  • General notes

     This product was previously labelled as Lymphotoxin Beta, Lymphotoxin Alpha + Beta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab64835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22354988
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional.
  • Tissue specificity

    Spleen and thymus.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • LT beta antibody
    • LT-beta antibody
    • Ltb antibody
    • lymphotoxin beta antibody
    • Lymphotoxin-beta antibody
    • p33 antibody
    • TNF-C antibody
    • TNFC antibody
    • TNFC_HUMAN antibody
    • TNFSF3 antibody
    • Tumor necrosis factor C antibody
    • Tumor necrosis factor ligand superfamily member 3 antibody
    see all

Images

  • All lanes : Anti-LTB antibody (ab64835) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
  • ab64835 at 1/500 - 1/1000 dilution staining Lymphotoxin Beta in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.

References

This product has been referenced in:

  • Yim RL  et al. Methylation of miR-155-3p in mantle cell lymphoma and other non-Hodgkin's lymphomas. Oncotarget 5:9770-82 (2014). Flow Cyt . Read more (PubMed: 25211095) »
  • Simonin Y  et al. Lymphotoxin signaling is initiated by the viral polymerase in HCV-linked tumorigenesis. PLoS Pathog 9:e1003234 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23555249) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (BCR-ABL cell line)
Permeabilization
No
Gating Strategy
gated on DAPI-(live)cells, and then gated on hCD4+ cells (cells transduced with EV or LTb)
Specification
BCR-ABL cell line
Preparation
Cell harvesting/tissue preparation method: cells are transduced with either empty vector (pMSCV-hCD4)or Lymphotoxin beta. cultured for > 48h for stable expression. The transduced cells are stained with ab64835, and than stained with anti-rabbit-AF647(Jacksonimmunoresearch).
Sample buffer: 20% FBS IMDM medium for culture. FACS buffer for staining.
Fixation
no fixation

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Verified customer

Submitted Oct 16 2018

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