Key features and details
- Rabbit polyclonal to LTB
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LTB antibody
See all LTB primary antibodies
DescriptionRabbit polyclonal to LTB
Specificityab64835 detects endogenous levels of total Lymphotoxin Beta protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human LTB (C terminal).
This product was previously labelled as Lymphotoxin Beta, Lymphotoxin Alpha + Beta
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
FunctionCytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional.
Tissue specificitySpleen and thymus.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- LT beta antibody
- LT-beta antibody
- Ltb antibody
All lanes : Anti-LTB antibody (ab64835) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 25 kDa
Observed band size: 25 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
ab64835 at 1/500 - 1/1000 dilution staining Lymphotoxin Beta in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
ab64835 has been referenced in 3 publications.
- Yim RL et al. Methylation of miR-155-3p in mantle cell lymphoma and other non-Hodgkin's lymphomas. Oncotarget 5:9770-82 (2014). Flow Cyt . PubMed: 25211095
- Simonin Y et al. Lymphotoxin signaling is initiated by the viral polymerase in HCV-linked tumorigenesis. PLoS Pathog 9:e1003234 (2013). WB, IHC-P ; Mouse . PubMed: 23555249
- Watanabe T et al. Higher-order chromatin regulation and differential gene expression in the human tumor necrosis factor/lymphotoxin locus in hepatocellular carcinoma cells. Mol Cell Biol 32:1529-41 (2012). IHC-P ; Human . PubMed: 22354988