Product nameAnti-LTBR antibody
See all LTBR primary antibodies
DescriptionRabbit polyclonal to LTBR
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human LTBR aa 385-435. The exact sequence is proprietary.
Database link: P36941
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226377 was affinity purified using an epitope specific to LTBR immobilized on solid support.
Our Abpromise guarantee covers the use of ab226377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 47 kDa.|
FunctionReceptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
Sequence similaritiesContains 4 TNFR-Cys repeats.
- Information by UniProt
- CD18 antibody
- D12S370 antibody
- LT beta R antibody
All lanes : Anti-LTBR antibody (ab226377) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
LTBR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226377 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226377 at 0.4 µg/ml.
Lane 1: ab226377 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226377 has not yet been referenced specifically in any publications.