Overview

  • Product name

  • Description

    Rabbit polyclonal to LTBR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human LTBR.

  • Positive control

    • HeLa cells and extract, HepG2 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab70063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD18 antibody
    • D12S370 antibody
    • LT beta R antibody
    • LTBETAR antibody
    • Ltbr antibody
    • Lymphotoxin B receptor antibody
    • Lymphotoxin beta receptor (TNFR superfamily, member 3) antibody
    • Lymphotoxin beta receptor antibody
    • Lymphotoxin-beta receptor antibody
    • TNF R III antibody
    • TNF-RIII antibody
    • TNFCR antibody
    • TNFR RP antibody
    • TNFR superfamily member 3 antibody
    • TNFR-III antibody
    • TNFR2 RP antibody
    • TNFR2RP antibody
    • TNFR3 antibody
    • TNFRII antibody
    • TNFRRP antibody
    • TNFRSF 3 antibody
    • TNFRSF3 antibody
    • TNR3_HUMAN antibody
    • Tumor necrosis factor C receptor antibody
    • Tumor necrosis factor receptor 2 related protein antibody
    • Tumor necrosis factor receptor 2-related protein antibody
    • Tumor necrosis factor receptor superfamily member 3 antibody
    • Tumor necrosis factor receptor superfamily member 3 precursor antibody
    • Tumor necrosis factor receptor type III antibody
    see all

Images

  • All lanes : Anti-LTBR antibody (ab70063) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : HeLa cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 48 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.

  • HeLa cells with ab70063 at 1/500 dilution, with and without the immunising peptide.

References

This product has been referenced in:

  • Shen M  et al. Lymphotoxin ß receptor activation promotes bladder cancer in a nuclear factor-?B-dependent manner. Mol Med Rep 11:783-90 (2015). IHC-P ; Human . Read more (PubMed: 25369740) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (monocytes)
Loading amount
20 µg
Specification
monocytes
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.6% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 26 2012

Answer

Thank you for your enquiry. I can confirm that Anti-LTBR antibody (ab70063) has been tested and guaranteed in human samples. For mouse samples, predicted to react means that the mouse sequence has high alignment to the human immunogen sequence used, and so the antibody is likely to detect in mouse. However, I am sorry that regrettably it has not been tested and cannot be guaranteed for mouse . If you would like to test the antibody in mouse, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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