Overview

  • Product name

    Anti-LTV1 antibody
  • Description

    Rabbit polyclonal to LTV1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: LEGSIQVDSN RLQEVLNDYY KEKAENCVKL NTLEPLEDQD LPMNELDESE EEEMITVVLE EAKEKWDCES ICSTYSNLYN HPQLIKYQPK PKQ, corresponding to amino acids of Human LTV1.

  • Positive control

    • Human placenta tissue; RT 4, U 251 MG and Human tonsil lysates; U 251MG cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Sequence similarities

    Belongs to the LTV1 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • C6orf93 antibody
    • Chromosome 6 open reading frame 93 antibody
    • dJ468K18.4 antibody
    • FLJ14909 antibody
    • FLJ18519 antibody
    • Ltv1 antibody
    • LTV1 homolog (S. cerevisiae) antibody
    • LTV1_HUMAN antibody
    • OTTHUMP00000017340 antibody
    • Protein LTV1 homolog antibody
    see all

Images

  • All lanes : Anti-LTV1 antibody (ab122100) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique.
  • ab122100, at 1/200, staining LTV1 in paraffin embedded Human placenta tissue by Immunohistochemistry.
  • ab122100, at 4 µg/ml, staining LTV1 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

References

This product has been referenced in:

  • Landry-Voyer AM  et al. Human PDCD2L Is an Export Substrate of CRM1 That Associates with 40S Ribosomal Subunit Precursors. Mol Cell Biol 36:3019-3032 (2016). Read more (PubMed: 27697862) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (neuroblastome)
Specification
neuroblastome
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 02 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up