Overview

  • Product name
  • Description
    Rabbit polyclonal to Lubricin
  • Host species
    Rabbit
  • Specificity
    ab28484 recognises Lubricin.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, African green monkey
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide:

    CLPNIRKQPDGYDYYAFSKDQ

    , corresponding to amino acids 1356-1374 of Human Lubricin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 156 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Lubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Articular superficial zone protein antibody
    • bG174L6.2 antibody
    • CACP antibody
    • Camptodactyly arthropathy coxa vara pericarditis syndrome gene antibody
    • FLJ32635 antibody
    • HAPO antibody
    • Jacobs camptodactyly arthropathy pericarditis syndrome gene antibody
    • JCAP antibody
    • Megakaryocyte stimulating factor antibody
    • MSF antibody
    • PRG 4 antibody
    • PRG4 antibody
    • Proteoglycan 4 antibody
    • Proteoglycan4 antibody
    • Superficial zone proteoglycan antibody
    • SZP antibody
    see all

Images

  • ab28484 (4µg/ml) staining lubricin in Human liver using an automated system. Using this protocol there is staining of the cytoplasm and nuclei in hepatic arteries and plates of hepatic cells.
    Sections were rehydrated and antigen retrieved with  buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked  for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected  for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ab28484 staining Lubricin in COS-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.25% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 568-conjugated goat anti-rabbit polyclonal, diluted 1/400.

    For permeabilization, 0.25% Triton X-100 was included in primary and secondary antibody incubation buffers.

    Cells were also stained with FITC-labeled Peanut Agglutinin. PNA binds lubricin and certain other glycoproteins, so some co-localization was expected between the Alexa Fluor 568 signal and the FITC signal.

    Unexpected co-localization was noted between the Alexa Fluor 568 staining (indirect lubricin staining) and DAPI (nuclear counterstain) in the cell nuclei.

    See Abreview

  • Anti-Lubricin antibody (ab28484) at 2 µg/ml + non-reduced human synovial fluid at 3 µg

    Predicted band size: 156 kDa



    Primary antibody was diluted in antibody diluent using a Fast Western Blot kit and followed by the optimized HRP reagent. Chemiluminescent detection was performed using SuperSignal West Dura.

References

This product has been referenced in:
  • Ji B  et al. Isoliquiritigenin blunts osteoarthritis by inhibition of bone resorption and angiogenesis in subchondral bone. Sci Rep 8:1721 (2018). Read more (PubMed: 29379010) »
  • Ng JJ  et al. Recapitulation of physiological spatiotemporal signals promotes in vitro formation of phenotypically stable human articular cartilage. Proc Natl Acad Sci U S A 114:2556-2561 (2017). IHC-P ; Human . Read more (PubMed: 28228529) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (cartilage)
Antigen retrieval step
None
Permeabilization
No
Specification
cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
zinc buffered formalin

Abcam user community

Verified customer

Submitted Feb 15 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (synovium)
Antigen retrieval step
None
Permeabilization
No
Specification
synovium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
zinc buffered formalin

Abcam user community

Verified customer

Submitted Feb 15 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (neuroepithelioma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
neuroepithelioma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 26 2015

Question
Answer

DISCOUNT CODE: XXXXXXX
Expiration date: 05-06-2013
Value: €380

I am very pleased to hear you would like to accept our offer and test ab28484 in Prcine. This code will give you $380 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Porcine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer


I am very pleased to hear you would like to test ab28484 in IHC. This code will give you $325 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Answer

Thank you for your reply.

Does the antibody still work in IHC? The reason I ask is that the last order from Cornell for this product is from September 2011. We do offer testing discounts for customers interested in testing our products in untested species or applications, but this is usually set up before the order is placed as part of our AbTrial program.

https://www.abcam.com/index.html?pageconfig=resource&rid=11998

If the antibody is still working in IHC, I can register you in the AbTrial program where you could test the antibody in ELISA, submit the results to us in the form of an Abreview, and receive a discount worth the price you paid for the antibody (so $325) off your next Abcam purchase within 4 months.

Please let me know how you would like to proceed. I look forward to your reply.

Read More

Answer

Thank you for contacting us.

The only other anti-lubricin antibody that we carry is ab94933 which is tested in IHC-P and WB.

https://www.abcam.com/lubricin-antibody-ab94933.html

Unfortunately we do not carry an anti-lubricin antibody that has been tested in ELISA. If you already have the ab28484, you could try it in ELISA as it may very well work, we just haven't tested it in ELISA.

If you do try it in ELISA, I'd encourage you to submit an Abreview letting us know whether or not it works and you could earn some Abpoints.

https://www.abcam.com/index.html?pageconfig=resource&rid=10332

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab28484 with the order number 1039188.
To check the status of the order please contact our Customer Service team and reference this number.
**As we discussed, when you have a chance please submit an Abreview for ab28484 relating your experience using this antibody in ELISA. For more information regarding our review system please see: https://www.abcam.com/abreviews
Thanks very much!

Read More

Answer

Thank you for you enquiry. I have contacted the lab which supplies ab28484 and unfortunately the blocking peptide for that antibody is not available. Instead of using a blocking peptide for a negative control, I would recommend a sample that you know does not contain lubricin. This would be an even better control than the blocking peptide. I hope this is helpful. Please contact me again if you need anything further.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (COS-1 line, from african green monkey kidney)
Specification
COS-1 line, from african green monkey kidney
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Ms. Melinda Stivers

Verified customer

Submitted Sep 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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