Product nameAnti-Lubricin antibody
See all Lubricin primary antibodies
DescriptionRabbit polyclonal to Lubricin
Specificityab28484 recognises Lubricin.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, African green monkey
Predicted to work with: Horse
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 156 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevanceLubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.
- Articular superficial zone protein antibody
- bG174L6.2 antibody
- CACP antibody
ab28484 (4µg/ml) staining lubricin in Human liver using an automated system. Using this protocol there is staining of the cytoplasm and nuclei in hepatic arteries and plates of hepatic cells.
Sections were rehydrated and antigen retrieved with buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab28484 staining Lubricin in COS-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.25% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 568-conjugated goat anti-rabbit polyclonal, diluted 1/400.
For permeabilization, 0.25% Triton X-100 was included in primary and secondary antibody incubation buffers.
Cells were also stained with FITC-labeled Peanut Agglutinin. PNA binds lubricin and certain other glycoproteins, so some co-localization was expected between the Alexa Fluor 568 signal and the FITC signal.
Unexpected co-localization was noted between the Alexa Fluor 568 staining (indirect lubricin staining) and DAPI (nuclear counterstain) in the cell nuclei.
Anti-Lubricin antibody (ab28484) at 2 µg/ml + non-reduced human synovial fluid at 3 µg
Predicted band size: 156 kDa
Primary antibody was diluted in antibody diluent using a Fast Western Blot kit and followed by the optimized HRP reagent. Chemiluminescent detection was performed using SuperSignal West Dura.
This product has been referenced in:
- Ji B et al. Isoliquiritigenin blunts osteoarthritis by inhibition of bone resorption and angiogenesis in subchondral bone. Sci Rep 8:1721 (2018). Read more (PubMed: 29379010) »
- Ng JJ et al. Recapitulation of physiological spatiotemporal signals promotes in vitro formation of phenotypically stable human articular cartilage. Proc Natl Acad Sci U S A 114:2556-2561 (2017). IHC-P ; Human . Read more (PubMed: 28228529) »