Anti-Lubricin/MSF antibody (ab28484)
Key features and details
- Rabbit polyclonal to Lubricin/MSF
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Lubricin/MSF antibody
See all Lubricin/MSF primary antibodies -
Description
Rabbit polyclonal to Lubricin/MSF -
Host species
Rabbit -
Specificity
ab28484 recognises Lubricin/MSF.
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Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Horse -
Immunogen
Synthetic peptide corresponding to Human Lubricin/MSF aa 1356-1374.
Sequence:CLPNIRKQPDGYDYYAFSKDQ
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 156 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 156 kDa). |
Target
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Relevance
28484 is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of 28484 function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 10216 Human
- Entrez Gene: 96875 Mouse
- Omim: 604283 Human
- SwissProt: Q92954 Human
- SwissProt: Q9JM99 Mouse
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Alternative names
- Articular superficial zone protein antibody
- bG174L6.2 antibody
- CACP antibody
see all
Images
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Anti-Lubricin/MSF antibody (ab28484) at 2 µg/ml + non-reduced human synovial fluid at 3 µg
Predicted band size: 156 kDaPrimary antibody was diluted in antibody diluent using a Fast Western Blot kit and followed by the optimized HRP reagent. Chemiluminescent detection was performed using SuperSignal West Dura.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (50)
ab28484 has been referenced in 50 publications.
- Wei Y et al. EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression. J Bone Miner Res 37:1012-1023 (2022). PubMed: 35191092
- Fan C et al. A Cd9+Cd271+ stem/progenitor population and the SHP2 pathway contribute to neonatal-to-adult switching that regulates tendon maturation. Cell Rep 39:110762 (2022). PubMed: 35476985
- Armaka M et al. Single-cell multimodal analysis identifies common regulatory programs in synovial fibroblasts of rheumatoid arthritis patients and modeled TNF-driven arthritis. Genome Med 14:78 (2022). PubMed: 35879783
- Nagata K et al. Runx2 and Runx3 differentially regulate articular chondrocytes during surgically induced osteoarthritis development. Nat Commun 13:6187 (2022). PubMed: 36261443
- Wei Y et al. The critical role of Hedgehog-responsive mesenchymal progenitors in meniscus development and injury repair. Elife 10:N/A (2021). PubMed: 34085927