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I was wondering if you have a blocking peptide for anti-lubricin antibody (ab28484)? We have used this Ab in our Western blotting and Confocal microscopic experiments. The Ab recognized multiple bands on the blots including the predicted 280kDa one and stained not only the lubricin in extracellular matrix but something inside the nuclei. With the help of the blocking peptide, we could probably identify if there are non-specific bindings. Thanks!
Asked on Sep 28 2011
Thank you for you enquiry. I have contacted the lab which supplies ab28484 and unfortunately the blocking peptide for that antibody is not available. Instead of using a blocking peptide for a negative control, I would recommend a sample that you know does not contain lubricin. This would be an even better control than the blocking peptide. I hope this is helpful. Please contact me again if you need anything further.
Answered on Sep 28 2011