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I have used a few different vials of this antibody as a detection antibody for ELISA. It has previously worked well but this lot GR55635-1 produced very high background. In control wells, containing no target protein but only blocker, adding the primary produced OD values of about 1.0 but adding only the conjugated secondary produced no background.
Asked on Feb 22 2012
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab28484 with the order number 1039188.
To check the status of the order please contact our Customer Service team and reference this number.
**As we discussed, when you have a chance please submit an Abreview for ab28484 relating your experience using this antibody in ELISA. For more information regarding our review system please see: https://www.abcam.com/abreviews
Thanks very much!
Answered on Feb 22 2012