Key features and details
- Detection method: Luminescent
- Platform: Microplate
- Sample type: Adherent cells
Product nameLuciferase Reporter Assay Kit
Sample typeAdherent cells
Assay durationMultiple steps standard assay
Luciferase Reporter Assay Kit ab287865 (previously known as Luciferase Reporter Assay Kit K801) provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis, then CoA, ATP, Mg2+ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg2+ in an optimized buffer solution, this kit ensures maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.
This product is manufactured by BioVision, an Abcam company and was previously called K801 Luciferase Reporter Assay Kit. K801-200 is the same size as the 200 test size of ab287865.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Cell Lysis buffer 1 x 100ml Substrate A 1 vial Substrate B 1 vial
RelevanceLuciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
- ec 220.127.116.11
- Luciferin 4 monooxygenase
ab287865 has not yet been referenced specifically in any publications.