Product nameAnti-Lumican antibody
See all Lumican primary antibodies
DescriptionMouse polyclonal to Lumican
SpecificityThis antibody is reactive against mammalian transfected lysate.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit
Lumican (NP_002336, 1 a.a. ~ 338 a.a) full-length human protein.
- Transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab70191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa).|
Tissue specificityCornea and other tissues.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
Developmental stagePresent in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
modificationsSulfated on tyrosine residue(s).
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
All lanes : Anti-Lumican antibody (ab70191) at 1/500 dilution
Lane 1 : Lumican transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 38 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Rønning SB et al. The role of extracellular matrix components in pin bone attachments during storage-a comparison between farmed Atlantic salmon (Salmo salar) and cod (Gadus morhua L.). Fish Physiol Biochem 43:549-562 (2017). Read more (PubMed: 27807712) »
- Li X et al. Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth. Oncogene 36:5432-5438 (2017). WB . Read more (PubMed: 28534517) »