Key features and details
- Mouse polyclonal to Lumican
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lumican antibody
See all Lumican primary antibodies
DescriptionMouse polyclonal to Lumican
SpecificityThis antibody is reactive against mammalian transfected lysate.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit
Lumican (NP_002336, 1 a.a. ~ 338 a.a) full-length human protein.
- Transfected 293T cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab70191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa).|
Tissue specificityCornea and other tissues.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
Developmental stagePresent in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
modificationsSulfated on tyrosine residue(s).
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
All lanes : Anti-Lumican antibody (ab70191) at 1/500 dilution
Lane 1 : Lumican transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 38 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ab70191 has been referenced in 4 publications.
- Rønning SB et al. The role of extracellular matrix components in pin bone attachments during storage-a comparison between farmed Atlantic salmon (Salmo salar) and cod (Gadus morhua L.). Fish Physiol Biochem 43:549-562 (2017). PubMed: 27807712
- Li X et al. Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth. Oncogene 36:5432-5438 (2017). WB . PubMed: 28534517
- Gao Q et al. Altered protein expression profiles in umbilical veins: insights into vascular dysfunctions of the children born after in vitro fertilization. Biol Reprod 91:71 (2014). PubMed: 25100710
- Pedersen ME et al. Small leucine-rich proteoglycans in the vertebrae of Atlantic salmon Salmo salar. Dis Aquat Organ 106:57-68 (2013). PubMed: 24062553