Anti-Lumican antibody (ab70191)
Key features and details
- Mouse polyclonal to Lumican
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lumican antibody
See all Lumican primary antibodies -
Description
Mouse polyclonal to Lumican -
Host species
Mouse -
Specificity
This antibody is reactive against mammalian transfected lysate. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit -
Immunogen
Lumican (NP_002336, 1 a.a. ~ 338 a.a) full-length human protein.
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Positive control
- Transfected 293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa). |
Target
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Tissue specificity
Cornea and other tissues. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Developmental stage
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
Post-translational
modificationsSulfated on tyrosine residue(s). -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4060 Human
- Omim: 600616 Human
- SwissProt: P51884 Human
- Unigene: 406475 Human
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Alternative names
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
see all
Images
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All lanes : Anti-Lumican antibody (ab70191) at 1/500 dilution
Lane 1 : Lumican transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 38 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (4)
ab70191 has been referenced in 4 publications.
- Rønning SB et al. The role of extracellular matrix components in pin bone attachments during storage-a comparison between farmed Atlantic salmon (Salmo salar) and cod (Gadus morhua L.). Fish Physiol Biochem 43:549-562 (2017). PubMed: 27807712
- Li X et al. Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth. Oncogene 36:5432-5438 (2017). WB . PubMed: 28534517
- Gao Q et al. Altered protein expression profiles in umbilical veins: insights into vascular dysfunctions of the children born after in vitro fertilization. Biol Reprod 91:71 (2014). PubMed: 25100710
- Pedersen ME et al. Small leucine-rich proteoglycans in the vertebrae of Atlantic salmon Salmo salar. Dis Aquat Organ 106:57-68 (2013). PubMed: 24062553