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Hi, My customer is working on her research using IHC on rabbit corneal tissue. Hence, I would like to know if the following antibodies has high sequence homology to rabbit or if you have any latest data that shows they can cross-react with rabbit tissue? 1. ab70191 Mo anti-Lumican 2. ab56777 Mo anti-ALDH1L1 3. ab56305 Mo anti-MMP12 4. ab6328 Mo anti-Fibronectin Looking forward to your soonest reply. Thank you.
Asked on Nov 24 2011
Thank you very much for your interest in our antibodies. The immunogen and epitope sequences, respectively, show the following similarities against rabbit: 1) ab70191: 93% 2) ab56777: 96% 3) ab56305: 72% 4) ab6328: 100% Due to this high homology, the anti-Lumican antibody, the anti-ALDH1L1 antibody and the anti-Fibronectin antibody are likely to cross-react with the rabbit orthologs, whereas the homology for the MMP12 antibody seems to low which is why we would not recommend testing this antibody. However, if requested I would be pleased to look for another anti-MMP12 antibody that might be suitable for rabbit, as we have for example antibody against the N- or C-terminus of the protein. To our knowledge, ab70191, ab56777 and ab6328 have not been tested in rabbit yet. Therefore, I can offer a discount off a future purchase if you buy ab70191, ab56777 and/or ab6328 now, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody each. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab70191, ab56777 and/or ab6328 in rabbit. I will then send a discount code. This code must be issued before purchasing ab70191, ab56777 and/or ab6328 so please wait for my reply before ordering. 2. Purchase ab either by phone, fax, or online (www.abcam.com). 3. Test it in rabbit. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. Each discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab70191, ab56777 and/or ab6328 turn out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Answered on Nov 24 2011