Overview

  • Product name

  • Description

    Rabbit polyclonal to Lumican
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Lumican aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115236)

  • Positive control

    • Recombinant Human Lumican protein (ab114635) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: Liver (Human) Tissue Lysate. This antibody gave a positive signal in the following formaldehyde fixed cell lines: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab98067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificity

    Cornea and other tissues.
  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Developmental stage

    Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
  • Post-translational
    modifications

    Sulfated on tyrosine residue(s).
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Keratan sulfate proteoglycan lumican antibody
    • KSPG lumican antibody
    • LDC antibody
    • LUM antibody
    • LUM_HUMAN antibody
    • Lumican antibody
    • Lumican precursor antibody
    • SLRR2D antibody
    see all

Images

  • All lanes : Anti-Lumican antibody (ab98067) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 50 kDa (possible glycosylated form), 60 kDa (possible glycosylated form)


    Exposure time: 8 minutes


    We hypothesise that the antibody recognises non-modified Lumican in human liver tissue lysate (lane 1). The bands around 50 and 60-kDa may represent glycosylated forms of the target protein. Lumican has 4 potential glycosylation sites (Swissprot data). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab98067 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98067 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Sharif R  et al. Collagen cross-linking impact on keratoconus extracellular matrix. PLoS One 13:e0200704 (2018). Read more (PubMed: 30020979) »
  • Kang Y  et al. Transforming Growth Factor-ß Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors. Clin Cancer Res 22:4934-4946 (2016). Read more (PubMed: 27126993) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Oligodendrocyte precursor cell line)
Permeabilization
Yes - 0.5%
Specification
Oligodendrocyte precursor cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Sueli Marques

Verified customer

Submitted Jan 12 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up