This antibody gave a positive signal in the following whole cell lysates: Liver (Human) Tissue Lysate.
This antibody gave a positive signal in the following formaldehyde fixed cell lines: HeLa cells.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Use a concentration of 1 µg/ml.
Use a concentration of 5 µg/ml.
Cornea and other tissues.
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily. Contains 11 LRR (leucine-rich) repeats. Contains 1 LRRNT domain.
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
Sulfated on tyrosine residue(s).
Secreted > extracellular space > extracellular matrix.
We hypothesise that the antibody recognises non-modified Lumican in human liver tissue lysate (lane 1). The bands around 50 and 60-kDa may represent glycosylated forms of the target protein. Lumican has 4 potential glycosylation sites (Swissprot data). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab98067 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98067 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Gu G et al. Lumican as a novel potential clinical indicator for acute aortic dissection: A comparative study, based on multi-slice computed tomography angiography. Exp Ther Med11:923-928 (2016).
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