Product nameAnti-Lumican antibody
See all Lumican primary antibodies
DescriptionRabbit polyclonal to Lumican
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow
Synthetic peptide corresponding to Human Lumican aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Lumican protein (ab114635) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: Liver (Human) Tissue Lysate. This antibody gave a positive signal in the following formaldehyde fixed cell lines: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab98067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|ELISA||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
Tissue specificityCornea and other tissues.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
Developmental stagePresent in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
modificationsSulfated on tyrosine residue(s).
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
All lanes : Anti-Lumican antibody (ab98067) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 50 kDa (possible glycosylated form), 60 kDa (possible glycosylated form)
Exposure time: 8 minutes
We hypothesise that the antibody recognises non-modified Lumican in human liver tissue lysate (lane 1). The bands around 50 and 60-kDa may represent glycosylated forms of the target protein. Lumican has 4 potential glycosylation sites (Swissprot data). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab98067 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98067 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Sharif R et al. Collagen cross-linking impact on keratoconus extracellular matrix. PLoS One 13:e0200704 (2018). Read more (PubMed: 30020979) »
- Kang Y et al. Transforming Growth Factor-ß Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors. Clin Cancer Res 22:4934-4946 (2016). Read more (PubMed: 27126993) »