Recombinant
RabMAb

Recombinant Anti-Lumican antibody [EPR8898(2)] - BSA and Azide free (ab198974)

Overview

  • Product name

    Anti-Lumican antibody [EPR8898(2)] - BSA and Azide free
    See all Lumican primary antibodies
  • Description

    Rabbit monoclonal [EPR8898(2)] to Lumican - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Lumican. The exact sequence is proprietary.
    Database link: P51884

  • Positive control

    • Saos-2, fetal skin, fetal heart, Jurkat, Y79 and 293T lysates , Human kidney and Human skin tissues, Saos-2 and 293T cells
  • General notes

    Ab198974 is the carrier-free version of ab168348. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab198974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Cornea and other tissues.
    • Sequence similarities

      Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
      Contains 11 LRR (leucine-rich) repeats.
      Contains 1 LRRNT domain.
    • Developmental stage

      Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
    • Post-translational
      modifications

      Sulfated on tyrosine residue(s).
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Keratan sulfate proteoglycan lumican antibody
      • KSPG lumican antibody
      • LDC antibody
      • LUM antibody
      • LUM_HUMAN antibody
      • Lumican antibody
      • Lumican precursor antibody
      • SLRR2D antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168348).

    • Flow Cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168348).

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168348).

    • This ICC/IF data was generated using the same anti-Lumican antibody clone, EPR8898(2), in a different buffer formulation (cat# ab168348).

      Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.

    References

    This product has been referenced in:

    • Zheng Z  et al. Fibromodulin-deficiency alters temporospatial expression patterns of transforming growth factor-ß ligands and receptors during adult mouse skin wound healing. PLoS One 9:e90817 (2014). IHC-P ; Mouse . Read more (PubMed: 24603701) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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