Product nameAnti-Lumican antibody [EPR8898(2)] (HRP)
See all Lumican primary antibodies
DescriptionRabbit monoclonal [EPR8898(2)] to Lumican (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Lumican. The exact sequence is proprietary.
Database link: P51884
- WB: Saos-2, Jurkat, Y79 and HEK293 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab201344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 38 kDa).|
Tissue specificityCornea and other tissues.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
Developmental stagePresent in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
modificationsSulfated on tyrosine residue(s).
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
All lanes : Anti-Lumican antibody [EPR8898(2)] (HRP) (ab201344) at 1/5000 dilution
Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201344 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab201344 has not yet been referenced specifically in any publications.