Recombinant Anti-Luteinizing Hormone beta antibody [EPR8196] - BSA and Azide free (ab221493)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8196] to Luteinizing Hormone beta - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Luteinizing Hormone beta antibody [EPR8196] - BSA and Azide free
See all Luteinizing Hormone beta primary antibodies -
Description
Rabbit monoclonal [EPR8196] to Luteinizing Hormone beta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human pituitary lysate; Human pituitary tissue
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General notes
ab221493 is the carrier-free version of ab150416.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8196 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
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IHC-P |
Use at an assay dependent concentration.
For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa. |
IHC-P
Use at an assay dependent concentration. For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
Target
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Function
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. -
Tissue specificity
Pituitary gland. -
Involvement in disease
Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB) [MIM:152780]. Hypogonadism is characterized by infertility and pseudohermaphroditism. -
Sequence similarities
Belongs to the glycoprotein hormones subunit beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3972 Human
- Omim: 152780 Human
- SwissProt: P01229 Human
- Unigene: 154704 Human
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Alternative names
- CGB4 antibody
- CHORIONIC GONADOTROPIN, BETA POLYPEPTIDE 4 antibody
- hLHB antibody
see all
Images
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This data was developed using ab150416, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human pituitary tissue labelling Luteinizing Hormone beta with ab150416 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab221493 has not yet been referenced specifically in any publications.