Overview

  • Product name
    Luxol Fast Blue Stain Kit (Myelin Stain)
  • Product overview

    Luxol Fast Blue Stain Kit (Myelin Stain) ab150675 is designed for staining myelin/myelinated axons and Nissl bodies on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.


    Luxol Fast Blue staining protocol summary:
    - deparaffinze sections if necessary and hydrate in water
    - incubate in Luxol Fast Blue solution for 24 hr at room temp, or 2 hr at 60ºC
    - rinse in water
    - differentiate by dipping in lithium carbonate solution
    - differentiate further by dipping in alcohol reagent
    - rinse in water
    - incubate in Cresyl Echt Violet for 2-5 min
    - rinse in water
    - dehydrate in absolute alcohol
    - clear and mount


     


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Myelinated Fibers     Blue
    Nissil Substance Violet
    Nerve Cells Violet

    Control Tissue: Cerebral Cortex, Spinal Cord.

Properties

  • Components 100 tests
    Alcohol reagent (70%) 1 x 500ml
    Cresyl Echt Violet Solution 1 x 125ml
    Lithium Carbonate Solution 1 x 500ml
    Luxol Fast Blue Solution 1 x 125ml
  • Research areas
  • Relevance
    Myelin is produced by oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS). Defining the differences between CNS and PNS myelin may provide insights into the process of myelination and the pathogenesis of certain idiopathic diseases involving myelin such as multiple sclerosis, certain leukodystrophies, and other myelinopathies. Myelin is composed of about 80% lipid fat and about 20% protein. Some of the proteins that make up myelin are Myelin basic protein (MBP), Myelin oligodendrocyte glycoprotein (MOG) and Proteolipid protein (PLP). Myelin is made up primarily of a glycolipid called galactocerebroside. The intertwining of the hydrocarbon chains of sphingomyelin serve to strengthen the myelin sheath.
  • Cellular localization
    Integral membrane protein.
  • Alternative names
    • MOG
    • MOG IG 2
    • Myelin oligodendrocyte glycoprotein

Images

  • ab150675 Luxol Fast Blue Stain Kit (Myelin Stain) staining formalin-fixed-paraffin embedded mouse brain.

  • ab150675 (Luxol Fast Blue stain) staining myelin (blue) in formalin fixed paraffin embedded normal human cerebellum, counterstained with cresyl echt violet (purple).

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Staining using ab150675 - Luxol Fast Blue Stain Kit.

Protocols

References

This product has been referenced in:
  • Xiao R  et al. Enriched environment regulates thymocyte development and alleviates experimental autoimmune encephalomyelitis in mice. Brain Behav Immun 75:137-148 (2019). Read more (PubMed: 30287389) »
  • Menal MJ  et al. Alzheimer's Disease Mutant Mice Exhibit Reduced Brain Tissue Stiffness Compared to Wild-type Mice in both Normoxia and following Intermittent Hypoxia Mimicking Sleep Apnea. Front Neurol 9:1 (2018). Read more (PubMed: 29403429) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
It is important, that the parafinslides are thick, about 10 um. And that the objektglas is electrostatic treated, so that the slides stayes on the glass.
After 1 hour in 60 degrees owen, deparafinish slides in Tissue Clear 10 min, and end in 96% alcohol.
Place in Luxol over night.
Wash in 70% alcohol.
Wash in water.
Differentiate the blue colour in Lithium Carbonate, about 20 sek. Do not use the solution several times! It does not differentiate that good after some times.
Differentiate further in 95% alcohol, about 10 sek.
Wash in water.
Check the colour in a microscope, and see if it should be differentiated more.
Differentiate more, if nessesary.
Filtrate the Cresyl Violet, and place slides for 10-20 min. in the solution.
Wash quickly in 3 x 99% alcohol, to Xylene
Mount coverslides with Pertex.

Ms. Heidi Marie Paulsen

Verified customer

Submitted Oct 25 2018

Answer

Thank you for your inquiry.

After verification with the lab, they advise to use a coplin jar to perform the staining. Both Luxol and Cresyl Echt Violet can be reused however we haven't determined how many times it can be done.

I hope this information is useful. Do not hesitate to contact us if you have any other question.

Read More
9-22-2014
We had previously observed changes in expression of myelin basic protein in the prefrontal cortex (PFC) using western blot analysis (experimental manipulation withheld as these data are not published). We are interested in finding out the effect of the same experimental manipulation on myelination using an independent method.
Objective: optimize a method to quantify change in myelin in the grey matter areas in paraformaldehyde (PFA) fixed tissue
Experimental conditions:
1. Previous studies have used Luxol Fast Blue to quantify myelination in the striatal tissue, therefore we used striatal sections as our tissue control. Prefrontal cortex (PFC) sections were our regions of interest.
2. Since we were interested in quantifying intensity of Luxol stain as a measure of myelination, we did not want a counter-stain to interfere with our analysis. So we tried three combinations. Luxol staining alone, Luxol with Fast Red counter stain, and (Abcam provided kit) Luxol with Cresyl Violet counter stain.
3. Tissue - we used 40 micron thick, PFA fixed tissue sections for our assay optimization. Tissue were either mounted on glass slides, dried and then stained or the free floating tissues sections were used for staining.
4. Duration of staining: 2 hr, 6 hr, 12 hr and 24 hr staining at room temperature were tested to optimize duration of staining
Results and conclusions:
1. Free-floating staining did not work. Since this is an acetic acid based stain, our PFA fixed tissues shriveled when left in the solution for the time intervals tested. Staining when the sections were mounted on slides worked better.
2. Optimal Duration of staining was found to be 6 hrs
3. Staining worked beautifully for the white matter corpus callosum tracts in the sections (see images in the Striatum and PFC). Furthermore, both Fast Red and Cresyl Violet counter stains worked well. I personally prefer the Fast Red counter-stained images due to better contrast between myelin and cell bodies.
4. even with these optimized procedures, destaining was patchy in grey matter areas, particularly, in the cortical regions (see attached images for striatum as well as PFC). As a consequence, quantification of myelination between tissue sections will not be possible using Luxol Fast Blue staining.
We thank Abcam for offering us this opportunity to test their product to see if it was suitable for our purposes.

Dr. Sucharita Somkuwar

Verified customer

Submitted Sep 30 2014

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