Anti-LXN/TCI antibody (ab154744)
Key features and details
- Rabbit polyclonal to LXN/TCI
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LXN/TCI antibody
See all LXN/TCI primary antibodies -
Description
Rabbit polyclonal to LXN/TCI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human LXN/TCI aa 1-222.
Database link: Q9BS40 -
Positive control
- 293T, HeLa S3, Molt4 and mouse brain lysates; mouse embryonic stem cells D3; Human FaDu xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. -
Tissue specificity
Highly expressed in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain. -
Sequence similarities
Belongs to the protease inhibitor I47 (latexin) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 56925 Human
- Entrez Gene: 17035 Mouse
- Omim: 609305 Human
- SwissProt: Q9BS40 Human
- SwissProt: P70202 Mouse
- Unigene: 478067 Human
- Unigene: 2632 Mouse
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Alternative names
- ECI antibody
- Endogenous carboxypeptidase inhibitor antibody
- Latexin antibody
see all
Images
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All lanes : Anti-LXN/TCI antibody (ab154744) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-LXN/TCI antibody (ab154744) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed mouse embryonic stem cells D3 labeling LXN/TCI with ab154744 at 1/200 dilution. Lower panel is co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded FaDu xenograft labeling LXN/TCI with ab154744 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154744 has been referenced in 2 publications.
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404
- Goveia J et al. An Integrated Gene Expression Landscape Profiling Approach to Identify Lung Tumor Endothelial Cell Heterogeneity and Angiogenic Candidates. Cancer Cell 37:21-36.e13 (2020). PubMed: 31935371