Overview

Applications

Our Abpromise guarantee covers the use of ab28478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 50 kDa.

Target

  • Function
    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Images

  • Anti-LXR alpha antibody (ab28478) (at a dilution of 1/500) + mouse liver samples

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

References

This product has been referenced in:
  • Shu L  et al. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes. Nat Commun 8:14147 (2017). WB . Read more (PubMed: 28128199) »
  • Sharma M  et al. Lipoprotein (a) upregulates ABCA1 in liver cells via scavenger receptor-B1 through its oxidized phospholipids. J Lipid Res 56:1318-28 (2015). Read more (PubMed: 25852127) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2, 293T, MEF)
Loading amount
20 µg
Specification
HepG2, 293T, MEF
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C

Mr. Dongil Kim

Verified customer

Submitted Feb 01 2013

Answer

Thank you for taking the time to contact us. I am sorry to hear the customer has some concerns regarding the results from this antibody.

In answer to your question, the alignment of the immunogen of ab28478 with beta subunit is 26%. So it is unlikely that the band at 56 kDa is the beta subunit ofLXR.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB and mouse samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if youare also able toprovide an image,including molecular weight markers, which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.



Order Details

Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More

Answer

I'm sorry to hear you have been experiencing problems with ab28478 in western blotting. Thank you for providing your protocol details which enabled me to understand rapidly how you performed your experiment. I have a few suggestions detailed below which I hope will improve your signal, as well as one question: can you please confirm the species of your purified protein? If it is for example of human origin and not mouse the problem of no detection of this sample could be due to the fact that the immunogen is different from the human sequence and the antibody may not be able to recognise human protein. For your mouse endogenous samples, it may be that those are at low levels below the detection threshold of the antibody. I think the following suggestions of modifications will also help: -check that the extraction buffer is adequate for the nuclear extraction of the protein. It may be that a nuclear fractionation is necessary in your endogenous samples to concentrate the protein -please check that the protease inhibitors in your endogenous samples are fresh; check also that the protein is extracted in plenty of time (i.e leaving samples to lyse for 2-4hours under rotation/agitation at 4C) -block the membrane in 5% BSA rather than milk -incubate the antibody in TBST only overnight at 4C -incubate the secondary antibody in TBST only and check that it works well with other primary antibodies. I hope the tips above will help you. If you still have problems with the recombinant protein and it is of mouse origin please do not hesitate to contact me and I can arrange for a replacement vial to be sent you; if the recombinant protein is of human origin I recommend to run a positive control of mouse liver lysate to make sure the problem with endogenous protein is not due to low levels, Good luck with your next set of experiments,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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