Anti-LXR alpha antibody (ab3585)
Key features and details
- Rabbit polyclonal to LXR alpha
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LXR alpha antibody
See all LXR alpha primary antibodies -
Description
Rabbit polyclonal to LXR alpha -
Host species
Rabbit -
Specificity
Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta. -
Tested Applications & Species
Application Species ICC Human -
Immunogen
Synthetic peptide corresponding to Human LXR alpha aa 318-329.
Sequence:DFSYNREDFAKA
(Peptide available asab5021)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Human
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All applications |
Cow
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Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
|
Notes |
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ICC
Use at an assay dependent concentration. |
Target
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Function
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
Tissue specificity
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
- SwissProt: Q13133 Human
- Unigene: 438863 Human
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Alternative names
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry - Anti-LXR alpha antibody (ab3585)This image is courtesy of an Abreview submitted by Dr Mahesh Mathrubutham
ab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.
Protocols
Datasheets and documents
References (18)
ab3585 has been referenced in 18 publications.
- Li T et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
- Xu D et al. SIRT2 plays a novel role on progesterone, estradiol and testosterone synthesis via PPARs/LXRa pathways in bovine ovarian granular cells. J Steroid Biochem Mol Biol 185:27-38 (2019). PubMed: 30009951
- Yin J et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648
- Jain J et al. Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV). Viruses 11:N/A (2019). PubMed: 31242674
- Cheng Y et al. Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor a in cardiomyocytes. Exp Ther Med 16:1814-1824 (2018). PubMed: 30186406