Anti-LXR alpha antibody (ab3585)

Reviews (3)Q&A (6)References (18)
Immunocytochemistry - Anti-LXR alpha antibody (ab3585)
  • Immunocytochemistry - Anti-LXR alpha antibody (ab3585)
  • Immunocytochemistry - Anti-LXR alpha antibody (ab3585)

Key features and details

  • Rabbit polyclonal to LXR alpha
  • Suitable for: ICC
  • Reacts with: Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
All applications
Cow
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.

Target

  • Function

    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

  • Tissue specificity

    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Liver X receptor alpha antibody
      • LXR a antibody
      • LXRA antibody
      • NR1H3 antibody
      • NR1H3_HUMAN antibody
      • Nuclear receptor subfamily 1 group H member 3 antibody
      • Oxysterols receptor LXR alpha antibody
      • Oxysterols receptor LXR-alpha antibody
      • RLD 1 antibody
      • RLD1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-LXR alpha antibody (ab3585)
      Immunocytochemistry - Anti-LXR alpha antibody (ab3585)

      Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

    • Immunocytochemistry - Anti-LXR alpha antibody (ab3585)
      Immunocytochemistry - Anti-LXR alpha antibody (ab3585)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

    • Immunocytochemistry - Anti-LXR alpha antibody (ab3585)
      Immunocytochemistry - Anti-LXR alpha antibody (ab3585)This image is courtesy of an Abreview submitted by Dr Mahesh Mathrubutham

      ab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

      See Abreview

    References (18)

    Publishing research using ab3585? Please let us know so that we can cite the reference in this datasheet.

    ab3585 has been referenced in 18 publications.

    • Li T  et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
    • Xu D  et al. SIRT2 plays a novel role on progesterone, estradiol and testosterone synthesis via PPARs/LXRa pathways in bovine ovarian granular cells. J Steroid Biochem Mol Biol 185:27-38 (2019). PubMed: 30009951
    • Yin J  et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648
    • Jain J  et al. Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV). Viruses 11:N/A (2019). PubMed: 31242674
    • Cheng Y  et al. Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor a in cardiomyocytes. Exp Ther Med 16:1814-1824 (2018). PubMed: 30186406
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-9 of 9 Abreviews or Q&A

    Question

    I am confused by looking at your product sheet. On the first page under application notes, the recommended dilution for Western Blot is 1/300 then on the next page you give an example of a blot with the antibody and you site the dilution as 1/1000. Which one is the recommended dilution?

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    Answer



    The lab has tested the antibody at a 1:300 dilution thus it is our recommended dilution, however ab3585 has been used successfully at a 1:1000 dilution by another user who submitted an Abreview with their protocol and data (the image that you have seen). We suggest titrating the antibody to find the optimal dilution for your particular samples and protocol, and starting this titration with a 1:300 dilution.

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    Question

    Bonjour,
    je souhaiterais commander un anticorps anti-LXR.


    (Ref LXRalpha: ab 41902, ab 3585, ab 106464 et LXR beta : ab76983, ab24361)
    Vous en proposez plusieurs, mais les références associées citées sur Pubmed sont très rares, et les commentaires sur votre site sont parfois très négatives (anticorps qui ne marchent pas)..
    Serait il possible de recevoir des aliquots /échantillons afin de tester les anticorps que j’achèterai ensuite?
    bien cordialement

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    Answer

    Merci de nous avoir contactés.

    Garantie sur les produits Abcam: nos produitssont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (garantie Abpromise). Nous pouvons donc toujours résoudre un problème rencontré avec un de nos produits en vous remboursant, ou vous envoyer un anticorps de remplacement (autre lot ou produit alternatif).
    L'anti-LXR alpha https://www.abcam.com/ab41902 par exemple a été testé etest doncgaranti pour fonctionner en ChIP, ELISA, Flow Cyt, GSA, IHC-P, IP, WB et chez les espèces suivantes : Homme, Souris, Rat.

    Informations sur les fiches techniques : nous indiquons sur les fiches techniques les applications et espèces qui ont été testées avec les produits, y compris celles qui ne sont pas compatibles avec le produit si nous avons cette information. Si l'information n'apparaît pas c'est que l'application ou l'espèce n'a pas été testée. Les espèces "predicted to react with" présentent une homologie de séquence égale ou supérieure à 90% mais elles n'ont pas encore été testées.

    Offre de test "100% testing discount" : notre catalogue contient plus de 70 000 références anticorps et nous ne pouvons malheureusement pas offrir, pour des raisons logistiques et de stockage, d'échantillon gratuit ou de petit volume pour des essais préliminaires.
    Cependant, si vous désirez tester un anticorps contre une espèce ou dans une application non testées préalablement, nous pouvons vous offrir une offre de test. Il suffit de contacter notre service scientifique en répondantà cetemail.
    Etapes à suivre :
    1. Nous confirmer que vous souhaiteriez tester un de nos anticorps dans une nouvelle espèce ou application afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de l'anticorps à tester
    2. Acheter l'anticorps par téléphone, fax ou internet (https://www.abcam.com/)
    3. Le tester dans une nouvelle espèce ou application
    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable pour la commande d'un anticorps primaire gratuit. Même si l'anticorps testé ne pas fonctionne pas dans l'application ou avec l'espèce testée, après soumission de votre Abreview, vous recevrez quand même un anticorps primaire gratuit.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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    Question

    He trabajado con LXR alpha (ab3585) y LXR beta antibody (ab76983) con resultados excelentes. Ahora que he enviado un paper me piden las referencias del péptido de bloqueo para confirmar la especificidad de los anticuerpos. A pesar de que obtenemos una única banda por WB, es necesario disponer de esta información. ¿Tenéis alguna referencia acerca del bloqueo del anticuerpo con el péptido tanto para LXR alpha (ab3585) como para LXR beta antibody (ab76983)?
    Muchas gracias.

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    Answer

    Muchas gracias por contactarnos de nuevo.
    Efectivamente el péptido usado para producir ab3585 es ab5021, y por tanto puede usarse en los ensayos de bloqueo del mismo. Sin embargo no hay ninguna publicación disponible acerca de este ensayo de bloqueo. El péptido para bloquear el anticuerpo contra LXR beta no está disponible tampoco, tampoco publicaciones al respecto.
    Lamento mucho no poder ayudar más. En cualquier caso estaré encanada de poder seguir ayudando.

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    Question

    I've been working with LXR alpha (ab3585) and LXR beta antibody (ab76983)with excellent results. Now, I submitted a paper and the referees asked me about the peptideto confirm the specificity of the antibodies...We have seen only one band at the expected size, but anyway, I was wondering if anyone has done already the resorption with the peptide? In other words, are there any references forLXR alpha peptide (ab5021)? and for LXR betapetide?

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    Answer

    Thank you for your inquiry.

    All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Therefore, unfortunately, if we do not have any references on our datasheet, we do not know about any. In addition, the epitope for the ab76983 Anti-LXR beta antibodyhas not been mapped.


    I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Western blot abreview for Anti-LXR alpha antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese Hamster Cell lysate - whole cell (CHO cell lysate)
    Loading amount
    50000 cells
    Specification
    CHO cell lysate
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
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    Abcam user community

    Verified customer

    Submitted Nov 21 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LXR alpha antibody

     Below Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa transiently expressing human LXRa.)
    Specification
    HeLa transiently expressing human LXRa.
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 21 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LXR alpha antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Hamster Cell (CHO-K1 cells expressing Human LXRa)
    Specification
    CHO-K1 cells expressing Human LXRa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1 % Triton X-100
    Blocking step
    TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3%
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    Dr. Mahesh Mathrubutham

    Verified customer

    Submitted Aug 21 2007

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band SAMPLE Brain Cortex Homogenated PRIMARY ANTIBODY - DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED - ANTIBODY STORAGE CONDITIONS -20ºC SAMPLE PREPARATION Protease Inhibitors AMOUNT OF PROTEIN LOADED 2 mg/ml, 14microL each band. ELECTROPHORESIS/GEL CONDITIONS Gel acrylamide 10% TRANSFER AND BLOCKING CONDITIONS - SECONDARY ANTIBODY - HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? - ADDITIONAL NOTES -

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    Answer

    Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Often it is possible to make suggestions to improve your results. If we determine that the antibody is not working as stated on the datasheet, and you have purchased it in the last 90 days, we would be happy to replace or refund the antibody for you. In order for me to help you further, can you please confirm the followings: 1) how was the sample prepared? was SDS and beta-mercaptoethenol used? did you boiled it and for how long? what lysis buffer did you used? did you add protease inhibitor? 2) can you confirm that you used 28micro grams of protein per lane? 3) what secondary antibody did you used, as there was no information in the questionnaire? was it a anti-rabbit antibody? 4) how long did you incubate the primary and secondary antibodies? what was the dilution and temperature? We recommend incubating the primary overnight, at 4oC. 5) did you perform blocking procedures? what was the duration, blocking buffer, and concentration used?We recommend using 5% BSA in TBST for 1 hr. Can you also please submit an image of the blot showing the non-specific bands? I am looking forward to receiving the answers to the above questions and also an image of the non-specific bands so that we can resolve the matter. Have a nice day.

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    Question

    Thanks very much for your so effeicent reply, besides we have some other questions about the products. First, can the antibodies for LXR alpha and LXR beta respectively(ab3585 - Rabbit polyclonal to LXR alpha ab24361 - Goat polyclonal to LXR beta) be applied to hamster or mouse? This information is very important for our research! Second, can the antibodies mentioned above be used in immunohisto chemistry ? Finally, we care about their price. Thank you very much and we are looking forward for your reply!

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    Answer

    Thank you for your email. For both of these antibodies, ab3585 and ab24361, they have only so been tested for cross-reactivity with human and have not yet been tested in other species. Ab24361 is predicted to react with Mouse (85% identity with immunogen) and Rat (92% identity with immunogen) due to sequence homology. Also, neither of these two antibodies have been tested yet for application in IHC. If you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). I'm not sure where you are located, but both of these antibodies are $299.00. To view the price for an antibody, just select your country from the drop-down menu at the top of the online datasheet. If you have any additional questions, please contact us again.

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