Overview

  • Product name
  • Description
    Rabbit polyclonal to LXR alpha
  • Host species
    Rabbit
  • Specificity
    Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese hamster
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human LXR alpha aa 318-329.
    Sequence:

    DFSYNREDFAKA


    (Peptide available as ab5021)

Properties

Applications

Our Abpromise guarantee covers the use of ab3585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/300. Can be blocked with LXR alpha peptide (ab5021).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22836489
EMSA Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

  • ab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-LXR alpha antibody (ab3585) at 1/1000 dilution + Whole cell lysate prepared from Chinese hamster CHO cells at 50000 cells

    Secondary
    HRP conjugated goat polyclonal to rabbit Ig at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

References

This product has been referenced in:
  • Rodriguez-Echevarria R  et al. Diet switch and omega-3 hydroxy-fatty acids display differential hepatoprotective effects in an obesity/nonalcoholic fatty liver disease model in mice. World J Gastroenterol 24:461-474 (2018). Read more (PubMed: 29398867) »
  • Mukwaya A  et al. Time-dependent LXR/RXR pathway modulation characterizes capillary remodeling in inflammatory corneal neovascularization. Angiogenesis 21:395-413 (2018). WB, IHC-P ; Rat . Read more (PubMed: 29445990) »
See all 13 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer



The lab has tested the antibody at a 1:300 dilution thus it is our recommended dilution, however ab3585 has been used successfully at a 1:1000 dilution by another user who submitted an Abreview with their protocol and data (the image that you have seen). We suggest titrating the antibody to find the optimal dilution for your particular samples and protocol, and starting this titration with a 1:300 dilution.

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Answer

Merci de nous avoir contactés.

Garantie sur les produits Abcam: nos produitssont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (garantie Abpromise). Nous pouvons donc toujours résoudre un problème rencontré avec un de nos produits en vous remboursant, ou vous envoyer un anticorps de remplacement (autre lot ou produit alternatif).
L'anti-LXR alpha https://www.abcam.com/ab41902 par exemple a été testé etest doncgaranti pour fonctionner en ChIP, ELISA, Flow Cyt, GSA, IHC-P, IP, WB et chez les espèces suivantes : Homme, Souris, Rat.

Informations sur les fiches techniques : nous indiquons sur les fiches techniques les applications et espèces qui ont été testées avec les produits, y compris celles qui ne sont pas compatibles avec le produit si nous avons cette information. Si l'information n'apparaît pas c'est que l'application ou l'espèce n'a pas été testée. Les espèces "predicted to react with" présentent une homologie de séquence égale ou supérieure à 90% mais elles n'ont pas encore été testées.

Offre de test "100% testing discount" : notre catalogue contient plus de 70 000 références anticorps et nous ne pouvons malheureusement pas offrir, pour des raisons logistiques et de stockage, d'échantillon gratuit ou de petit volume pour des essais préliminaires.
Cependant, si vous désirez tester un anticorps contre une espèce ou dans une application non testées préalablement, nous pouvons vous offrir une offre de test. Il suffit de contacter notre service scientifique en répondantà cetemail.
Etapes à suivre :
1. Nous confirmer que vous souhaiteriez tester un de nos anticorps dans une nouvelle espèce ou application afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de l'anticorps à tester
2. Acheter l'anticorps par téléphone, fax ou internet (https://www.abcam.com/)
3. Le tester dans une nouvelle espèce ou application
4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable pour la commande d'un anticorps primaire gratuit. Même si l'anticorps testé ne pas fonctionne pas dans l'application ou avec l'espèce testée, après soumission de votre Abreview, vous recevrez quand même un anticorps primaire gratuit.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Muchas gracias por contactarnos de nuevo.
Efectivamente el péptido usado para producir ab3585 es ab5021, y por tanto puede usarse en los ensayos de bloqueo del mismo. Sin embargo no hay ninguna publicación disponible acerca de este ensayo de bloqueo. El péptido para bloquear el anticuerpo contra LXR beta no está disponible tampoco, tampoco publicaciones al respecto.
Lamento mucho no poder ayudar más. En cualquier caso estaré encanada de poder seguir ayudando.

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Answer

Thank you for your inquiry.

All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Therefore, unfortunately, if we do not have any references on our datasheet, we do not know about any. In addition, the epitope for the ab76983 Anti-LXR beta antibodyhas not been mapped.


I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell lysate)
Loading amount
50000 cells
Specification
CHO cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 21 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa transiently expressing human LXRa.)
Specification
HeLa transiently expressing human LXRa.
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 21 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1 cells expressing Human LXRa)
Specification
CHO-K1 cells expressing Human LXRa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3%

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Answer

Thank you for your enquiry. I am sorry you are experiencing problems with this antibody. Often it is possible to make suggestions to improve your results. If we determine that the antibody is not working as stated on the datasheet, and you have purchased it in the last 90 days, we would be happy to replace or refund the antibody for you. In order for me to help you further, can you please confirm the followings: 1) how was the sample prepared? was SDS and beta-mercaptoethenol used? did you boiled it and for how long? what lysis buffer did you used? did you add protease inhibitor? 2) can you confirm that you used 28micro grams of protein per lane? 3) what secondary antibody did you used, as there was no information in the questionnaire? was it a anti-rabbit antibody? 4) how long did you incubate the primary and secondary antibodies? what was the dilution and temperature? We recommend incubating the primary overnight, at 4oC. 5) did you perform blocking procedures? what was the duration, blocking buffer, and concentration used?We recommend using 5% BSA in TBST for 1 hr. Can you also please submit an image of the blot showing the non-specific bands? I am looking forward to receiving the answers to the above questions and also an image of the non-specific bands so that we can resolve the matter. Have a nice day.

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Answer

Thank you for your email. For both of these antibodies, ab3585 and ab24361, they have only so been tested for cross-reactivity with human and have not yet been tested in other species. Ab24361 is predicted to react with Mouse (85% identity with immunogen) and Rat (92% identity with immunogen) due to sequence homology. Also, neither of these two antibodies have been tested yet for application in IHC. If you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). I'm not sure where you are located, but both of these antibodies are $299.00. To view the price for an antibody, just select your country from the drop-down menu at the top of the online datasheet. If you have any additional questions, please contact us again.

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