- Datasheet
- Specific References (13)
- Protocols
Overview
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Product nameAnti-LXR alpha antibody
See all LXR alpha primary antibodies -
DescriptionRabbit polyclonal to LXR alpha
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Host speciesRabbit
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SpecificityDetects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Human, Chinese hamster
Predicted to work with: Cow
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Immunogen
Synthetic peptide corresponding to Human LXR alpha aa 318-329.
Sequence:DFSYNREDFAKA
(Peptide available asab5021)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesLiver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab3585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/300. Can be blocked with LXR alpha peptide (ab5021). | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 22836489 | |
| EMSA | Use at an assay dependent concentration. |
Target
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FunctionOrphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
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Tissue specificityVisceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
see all -
Alternative names
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha antibody (ab3585)Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha antibody (ab3585)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha antibody (ab3585)This image is courtesy of an Abreview submitted by Dr Mahesh Mathrubuthamab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.
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Western blot - Anti-LXR alpha antibody (ab3585)This image is courtesy of an anonymous abreview.Anti-LXR alpha antibody (ab3585) at 1/1000 dilution + Whole cell lysate prepared from Chinese hamster CHO cells at 50000 cells
Secondary
HRP conjugated goat polyclonal to rabbit Ig at 1/1000 dilution
Performed under reducing conditions.
Observed band size: 50 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minute
Protocols
References
This product has been referenced in:
- Rodriguez-Echevarria R et al. Diet switch and omega-3 hydroxy-fatty acids display differential hepatoprotective effects in an obesity/nonalcoholic fatty liver disease model in mice. World J Gastroenterol 24:461-474 (2018). Read more (PubMed: 29398867) »
- Mukwaya A et al. Time-dependent LXR/RXR pathway modulation characterizes capillary remodeling in inflammatory corneal neovascularization. Angiogenesis 21:395-413 (2018). WB, IHC-P ; Rat . Read more (PubMed: 29445990) »
Customer reviews and Q&As
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