Non-Abcam antibody was used: Goat anti rabbit IgG with Alexa-568 from Invitroge Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor® 568
This Ab recognized an overexpressed LXRa in nucleus (upper right corner of the picture), not endogenous LXRa (other cells) with strong background signal. Longer blocking might have reduced a background. Not suitable for immunostaining with currrent protocol.
Thank you for you honest and thoughtful feedback. Based on a previous Abreview, I would recommend a longer incubation time with the primary (1 hour at RT), at a higher concentration 1/100. I would also recommend diluting the primary antibody in TBS + 3% dried milk + 0.1% Triton X-100, which can also be used as the block. This antibody does appear to need as longer blocking time, as the previous reviewer blocked for 20 hours.
Abcam user community
Submitted May 21 2008
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