Immunocytochemistry/ Immunofluorescence abreview for Anti-LXR alpha antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1 cells expressing Human LXRa)
Specification
CHO-K1 cells expressing Human LXRa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3%

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS + 3% dried milk + 0.1% Triton X-100

Secondary antibody

Name
Non-Abcam antibody was used: FITC labelled Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100

Additional data

Additional Notes
CHO-K1 cells expressing Human Liver X Receptor alpha (LXRa) clearly show nuclear localization of LXRa when grown under serum free conditions with the LXRa agonist, TO901317, prior to IF analysis.

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

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