Recombinant
RabMAb

Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Overview

  • Product name
    Anti-LXR alpha antibody [EPR6508(N)]
    See all LXR alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR6508(N)] to LXR alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide within Human LXR alpha aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13133
    (Peptide available as ab191835)

  • Positive control
    • Human fetal liver, Jurkat and MCF-7 cell lysates. Jurkat cells and MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab176323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.Can be blocked with LXR alpha peptide (ab191835).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
    • Tissue specificity
      Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
    • Sequence similarities
      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Liver X receptor alpha antibody
      • LXR a antibody
      • LXRA antibody
      • NR1H3 antibody
      • NR1H3_HUMAN antibody
      • Nuclear receptor subfamily 1 group H member 3 antibody
      • Oxysterols receptor LXR alpha antibody
      • Oxysterols receptor LXR-alpha antibody
      • RLD 1 antibody
      • RLD1 antibody
      see all

    Images

    • All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

      All lanes : Human SCC61 whole cell lysates

      Lysates/proteins at 23 µg per lane.

      Secondary
      All lanes : Rabbit anti-rabbit polyclonal HRP conjugate at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 50 kDa
      Additional bands at: 100 kDa (possible non-specific binding)


      Exposure time: 1 second


      From left to right lanes 1 - 3. Blocking was done with 5% milk in PBS incubated for 1 hour at 22°C.

      See Abreview

    • All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunofluorescent staining of Jurkat cells labeling LRX alpha using ab176323 at a 1/50 dilution.

    • Flow cytometric analysis of permeabilized MCF-7 cells labeling LXR alpha using ab176323 at a 1/10 dilution (red) or a rabbit IgG control (green).

    References

    This product has been referenced in:
    • Xie Z  et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29502100) »
    • Pan LL  et al. (-)-Epigallocatechin-3-Gallate Ameliorates Atherosclerosis and Modulates Hepatic Lipid Metabolic Gene Expression in Apolipoprotein E Knockout Mice: Involvement of TTC39B. Front Pharmacol 9:195 (2018). Read more (PubMed: 29593532) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    35 µg
    Specification
    Synovial membrane
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2016

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SCC61)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    23 µg
    Specification
    SCC61
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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