Western blot abreview for Anti-LXR alpha antibody [EPR6508(N)]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SCC61)
Gel Running Conditions
Reduced Denaturing
Loading amount
23 µg
Specification
SCC61
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Anti Rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 50 kDa Non-specific: 100 kDa

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Submitted Oct 15 2015

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