Western blot abreview for Anti-LXR alpha antibody [EPR6508(N)]

Excellent
Abreviews
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Synovial membrane)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Synovial membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 3% in TBST
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-linked whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
8 second(s)
Bands
Specific: 55 kDa Non-specific: 65 kDa
Positive control
Human THP-1 Cell Line

Additional data

Additional Notes
1. Rabbit Synovial membrane 2. Human THP-1 Cells Immunoglobulin light chain is observed at 65 KDa in rabbit samples

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Submitted Jun 09 2016

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