Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone Marrow-Derivated Macrophages from C57Bl6 mice)
Loading amount
40 µg
Specification
Bone Marrow-Derivated Macrophages from C57Bl6 mice
Treatment
T091317 ligand for 48 hours
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% BSA in TBS
Secondary antibody
Name
Non-Abcam antibody was used: a-murine IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Millipore
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
1: BMDM treated with DMSO
2: BMDM treated with T091317
2: BMDM treated with T091317
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jun 25 2010