Overview

  • Product name
    Anti-LXR alpha + LXR beta antibody
    See all LXR alpha + LXR beta primary antibodies
  • Description
    Goat polyclonal to LXR alpha + LXR beta
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Peptide with sequence (C)RLQDKKLPPLLSEI, from the C-terminus of the human protein sequences according to NP_005684; NP_009052.

  • Positive control
    • Human Spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab24362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Relevance
    LXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Liver X receptor alpha antibody
    • Liver X receptor beta antibody
    • LXR a antibody
    • LXR b antibody
    • LXRalpha antibody
    • LXRbeta antibody
    • NR1H2 antibody
    • NR1H3 antibody
    • Nuclear orphan receptor LXR alpha antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR beta antibody
    see all

Images

  • Anti-LXR alpha + LXR beta antibody (ab24362) at 1 µg/ml + Human spleen lysate(in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 hour


    Note: Additional minor bands consistently observed at 38kDa and 75kDa were successfully blocked by incubation with the immunising peptide.

References

This product has been referenced in:
  • Cruz-Garcia L & Schlegel A Lxr-driven enterocyte lipid droplet formation delays transport of ingested lipids. J Lipid Res 55:1944-58 (2014). Read more (PubMed: 25030662) »
  • Manna PR  et al. Mechanisms of action of hormone-sensitive lipase in mouse Leydig cells: its role in the regulation of the steroidogenic acute regulatory protein. J Biol Chem 288:8505-18 (2013). WB ; Mouse . Read more (PubMed: 23362264) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
40 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Mar 06 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab24362 in rabbit. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrabbit in WBand include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Cow Cell lysate - whole cell (muscle cells)
Loading amount
40 µg
Specification
muscle cells
Treatment
with LXR agonist for 24 hours
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C

Dr. GOSSELET Fabien

Verified customer

Submitted Nov 29 2010

Answer

Thank you for your enquiry. We have the following antibodies which may be of interest to you, please take a look at the online datasheets to see if there are applicable for your experiments (both have been tested in Western blotting): ab3585 - Rabbit polyclonal to LXR alpha ab24361 - Goat polyclonal to LXR beta If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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