Anti-LXR alpha + LXR beta antibody (ab24362)
Key features and details
- Goat polyclonal to LXR alpha + LXR beta
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LXR alpha + LXR beta antibody
See all LXR alpha + LXR beta primary antibodies -
Description
Goat polyclonal to LXR alpha + LXR beta -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish -
Immunogen
Peptide with sequence (C)RLQDKKLPPLLSEI, from the C-terminus of the human protein sequences according to NP_005684; NP_009052.
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Positive control
- WB: Human kidney and liver lysate; Flow Cyt (intra): A549 cells; ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (2) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Relevance
LXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 395221 Chicken
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Entrez Gene: 7376 Human
- Entrez Gene: 397553 Pig
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
see all -
Alternative names
- Liver X receptor alpha antibody
- Liver X receptor beta antibody
- LXR a antibody
see all
Images
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Immunocytochemical/Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% triton permeabilized A549 cells labelling LXR alpha + LXR beta with ab24362 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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All lanes : Anti-LXR alpha + LXR beta antibody (ab24362) at 1 µg/ml
Lane 1 : Human liver lysate in RIPA buffer
Lane 2 : Human kidney lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab24362 has been referenced in 7 publications.
- Li X et al. 27-hydroxycholesterol linked high cholesterol diet to lung adenocarcinoma metastasis. Oncogene 41:2685-2695 (2022). PubMed: 35379924
- Zhou W et al. Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice. Am J Transl Res 11:1498-1509 (2019). PubMed: 30972177
- Yang J et al. Circulating ectodysplasin A is a potential biomarker for nonalcoholic fatty liver disease. Clin Chim Acta 499:134-141 (2019). PubMed: 31526774
- Rather RA et al. Aqueous Terminalia arjuna extract modulates expression of key atherosclerosis-related proteins in a hypercholesterolemic rabbit: A proteomic-based study. Proteomics Clin Appl 10:750-9 (2016). PubMed: 26934842
- Cruz-Garcia L & Schlegel A Lxr-driven enterocyte lipid droplet formation delays transport of ingested lipids. J Lipid Res 55:1944-58 (2014). PubMed: 25030662
- Manna PR et al. Mechanisms of action of hormone-sensitive lipase in mouse Leydig cells: its role in the regulation of the steroidogenic acute regulatory protein. J Biol Chem 288:8505-18 (2013). WB ; Mouse . PubMed: 23362264
- Donmez G et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . PubMed: 20655472