Overview

  • Product name

    Anti-LXR beta/NER antibody
    See all LXR beta/NER primary antibodies
  • Description

    Goat polyclonal to LXR beta/NER
  • Host species

    Goat
  • Specificity

    Based on the peptide used, this antibody is expected to be specific for LXR beta/NER and not to cross-react with LXR alpha.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human LXR beta/NER aa 2-14 (N terminal).
    Sequence:

    SSPTTSSLDT PLPG(C)

  • Positive control

    • ICC/IF: U2OS cells FC: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab24361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Liver X nuclear receptor beta antibody
    • Liver X receptor beta antibody
    • LX receptor beta antibody
    • LXR b antibody
    • LXR beta antibody
    • LXRB antibody
    • NER antibody
    • NER I antibody
    • NER1 antibody
    • NR1H 2 antibody
    • NR1H2 antibody
    • NR1H2_HUMAN antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor NER antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • OR-1 antibody
    • Oxysterols receptor LXR-beta antibody
    • RIP15 antibody
    • Steroid hormone nuclear receptor NER antibody
    • Ubiquitously-expressed nuclear receptor antibody
    • UNR antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of U2OS cells labeling LXR beta/NER with ab24361 at 10μg/mL. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. The secondary antibody was Alexa Fluor®488 at 2μg/mL. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10μg/mL followed by Alexa Fluor®488 secondary antibody (2μg/mL). Nuclear statining is observed.

  • Flow cytometric analysis of HeLa cells labeling LXR beta/NER antibody with ab24361 at 10μg/mL (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Alexa Fluor®488 at 1μg/mL was used as secondary antibody. IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor®488 secondary antibody.

References

This product has been referenced in:

  • Grinman DY  et al. Liver X receptor-a activation enhances cholesterol secretion in lactating mammary epithelium. Am J Physiol Endocrinol Metab 316:E1136-E1145 (2019). Read more (PubMed: 30964702) »
  • Courtaut F  et al. Liver X receptor ligand cytotoxicity in colon cancer cells and not in normal colon epithelial cells depends on LXRß subcellular localization. Oncotarget 6:26651-62 (2015). IHC . Read more (PubMed: 26450852) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Merci de nous avoir contactés.

Garantie sur les produits Abcam: nos produitssont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (garantie Abpromise). Nous pouvons donc toujours résoudre un problème rencontré avec un de nos produits en vous remboursant, ou vous envoyer un anticorps de remplacement (autre lot ou produit alternatif).
L'anti-LXR alpha https://www.abcam.com/ab41902 par exemple a été testé etest doncgaranti pour fonctionner en ChIP, ELISA, Flow Cyt, GSA, IHC-P, IP, WB et chez les espèces suivantes : Homme, Souris, Rat.

Informations sur les fiches techniques : nous indiquons sur les fiches techniques les applications et espèces qui ont été testées avec les produits, y compris celles qui ne sont pas compatibles avec le produit si nous avons cette information. Si l'information n'apparaît pas c'est que l'application ou l'espèce n'a pas été testée. Les espèces "predicted to react with" présentent une homologie de séquence égale ou supérieure à 90% mais elles n'ont pas encore été testées.

Offre de test "100% testing discount" : notre catalogue contient plus de 70 000 références anticorps et nous ne pouvons malheureusement pas offrir, pour des raisons logistiques et de stockage, d'échantillon gratuit ou de petit volume pour des essais préliminaires.
Cependant, si vous désirez tester un anticorps contre une espèce ou dans une application non testées préalablement, nous pouvons vous offrir une offre de test. Il suffit de contacter notre service scientifique en répondantà cetemail.
Etapes à suivre :
1. Nous confirmer que vous souhaiteriez tester un de nos anticorps dans une nouvelle espèce ou application afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de l'anticorps à tester
2. Acheter l'anticorps par téléphone, fax ou internet (https://www.abcam.com/)
3. Le tester dans une nouvelle espèce ou application
4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable pour la commande d'un anticorps primaire gratuit. Même si l'anticorps testé ne pas fonctionne pas dans l'application ou avec l'espèce testée, après soumission de votre Abreview, vous recevrez quand même un anticorps primaire gratuit.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Answer

Thank you foryour message.

I can confirm that you will need to dilute the antibody before you use it in an experiment.

We recommend to only dilute the amount of antibody you need. The rest should be stored undiluted as recommended on the datasheet.

As stated on the datasheet, for WB we recommenda concentration of 1 -3 ug/ml. For IHC-P we recommend a concentration of 1 - 2 ug/ml. Please note, these dilutions are a guideline only and may require further optimizationby the end user.

As previously discussed, I am sorry we are not able to give you a price as we do not sell directly to India.Please contact one of the following distributors in India. They can provide a price to you:

Central and South India
Allied Scientific Products
39/19B Gopal Nagar Road
Kolkata-700027
India
Website
http://www.alliedscientific.net
Freephone 1800 345 7777
Fax+91 33 24566683 / 24790626
Tel+91 33 24566684/24793109/9039

Northern India

NuLife Consultants & Distributors (Pvt.) Ltd.
M-52, First Floor,
Lajpat Nagar- Part-II,
New Delhi-110024-India
Tel 011-46112691/ 46112692/ 29836141
Website
http://www.nulife.in
Fax 91 - 011-46586216
Emailmailto: nulife@bol.net.in
mailto:sales@nulife.in

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your message

As stated on the datasheet, the vial size is 100 µg. This is the only size we provide for this product.

As my customer service colleague has previously discussed with you, unfortunately, Abcam does not sell directly in India, However, we have to distributors that you can contact directly for further details. They will be able to provide you with a price. I can recommend to contact them:

Central and South India
Allied Scientific Products
39/19B Gopal Nagar Road
Kolkata-700027
India
Website
http://www.alliedscientific.net
Freephone 1800 345 7777
Fax+91 33 24566683 / 24790626
Tel+91 33 24566684/24793109/9039

Northern India
NuLife Consultants & Distributors (Pvt.) Ltd.
M-52, First Floor,
Lajpat Nagar- Part-II,
New Delhi-110024-India
Tel 011-46112691/ 46112692/ 29836141
Website
http://www.nulife.in
Fax 91 - 011-46586216
Emailmailto: nulife@bol.net.in
mailto:sales@nulife.in

They will be able to contact us onyourbehalf in order to provide atesting discount code if you would like to test this in IHC-Fr.

I hope this will be helpful. Please do not hesitate to let me know if you need any thing further.

Read More

Answer

Thank you for your enquiry and your interest in our products.

We have several antibodies against LXR beta (ab24361, ab56237, ab58089, ab58092, ab76983, ab106473) . The tested application/species/unit size can be found on the on-line product datasheets. I would advise you to take a look at these document to see which product would suit you need best.

If you need any further assistance in the future, please do not hesitate to contact me.

Regarding shipment and packaging information, could you please contact our authorized distributor in India for further details.

NuLife Consultants & Distributors (Pvt.) Ltd.
NuLife Consultants & Distributors (Pvt.) Ltd.
M-52, First Floor,
Lajpat Nagar- Part-II,
New Delhi-110024-India
Tel 011-46112691/ 46112692/ 29836141

Website http://www.nulife.in/

Fax 91 - 011-46586216 Email mailto:nulife@bol.net.inand mailto:sales@nulife.in

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Answer

Thank you for your email. For both of these antibodies, ab3585 and ab24361, they have only so been tested for cross-reactivity with human and have not yet been tested in other species. Ab24361 is predicted to react with Mouse (85% identity with immunogen) and Rat (92% identity with immunogen) due to sequence homology. Also, neither of these two antibodies have been tested yet for application in IHC. If you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). I'm not sure where you are located, but both of these antibodies are $299.00. To view the price for an antibody, just select your country from the drop-down menu at the top of the online datasheet. If you have any additional questions, please contact us again.

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