Anti-LXR beta/NER antibody (ab28479)
Key features and details
- Rabbit polyclonal to LXR beta/NER
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LXR beta/NER antibody
See all LXR beta/NER primary antibodies -
Description
Rabbit polyclonal to LXR beta/NER -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat LXR beta/NER aa 113-126.
Sequence:RYACRGSG TCQMDA
(Peptide available asab39817) -
Positive control
- WB: Hep G2, PC-3, 3T3-L1, HeLa, Mouse Lung, Mouse lung, Rat liver extracts. ICC/IF: C2C12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 51 kDa.
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| ICC/IF |
1/200 - 1/1000.
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| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 51 kDa. |
|
ICC/IF
1/200 - 1/1000. |
Target
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Function
Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7376 Human
- Entrez Gene: 22260 Mouse
- Entrez Gene: 58851 Rat
- Omim: 600380 Human
- SwissProt: P55055 Human
- SwissProt: Q60644 Mouse
- SwissProt: Q62755 Rat
- Unigene: 432976 Human
see all -
Alternative names
- Liver X nuclear receptor beta antibody
- Liver X receptor beta antibody
- LX receptor beta antibody
see all
Images
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Western blot - Anti-LXR beta/NER antibody (ab28479)All lanes : Anti-LXR beta/NER antibody (ab28479) at 2 µg/ml
Lane 1 : Hep G2 nuclear enriched extract
Lane 2 : PC-3 nuclear enriched extract
Lane 3 : 3T3-L1 nuclear enriched extract
Lane 4 : Mouse Lung tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution
Predicted band size: 51 kDaA 51 kDa band corresponding to LXR beta was observed across the cell lines and tissue tested.
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Western blot - Anti-LXR beta/NER antibody (ab28479)All lanes : Anti-LXR beta/NER antibody (ab28479) at 1/1000 dilution
Lane 1 : Rat liver cell lysate
Lane 2 : Mouse liver cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Predicted band size: 51 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-LXR beta/NER antibody (ab28479)ab28479 labelling LXR beta/NER (green) in the nuclear envelope of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-LXR beta/NER antibody (ab28479)ab28479 staining LXR beta/NER in C2C12 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue and F-actin stained red. Panel d shows the merged image
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab28479 has been referenced in 16 publications.
- Pu Y et al. MicroRNA-325 facilitates atherosclerosis progression by mediating the SREBF1/LXR axis via KDM1A. Life Sci 277:119464 (2021). PubMed: 33811891
- Yu W et al. Activation of LXRß Signaling in the Amygdala Confers Anxiolytic Effects Through Rebalancing Excitatory and Inhibitory Neurotransmission upon Acute Stress. Neurotherapeutics 17:1253-1270 (2020). PubMed: 32297184
- Desai R et al. Mitochondria form contact sites with the nucleus to couple prosurvival retrograde response. Sci Adv 6:N/A (2020). PubMed: 33355129
- Li T et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
- Fan W et al. Reduced Sirtuin1 signalling exacerbates diabetic mice hindlimb ischaemia injury and inhibits the protective effect of a liver X receptor agonist. J Cell Mol Med 24:5476-5490 (2020). PubMed: 32286000
