Overview

  • Product name
    Anti-LXR beta/NER antibody
    See all LXR beta/NER primary antibodies
  • Description
    Rabbit polyclonal to LXR beta/NER
  • Host species
    Rabbit
  • Specificity
    ab28479 recognises LXR beta/NER.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat LXR beta/NER aa 113-126.
    Sequence:

    RYACRGSG TCQMDA


    (Peptide available as ab39817)

  • Positive control
    • Mouse and rat liver

Properties

Applications

Our Abpromise guarantee covers the use of ab28479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 51 kDa.Can be blocked with Rat LXR beta/NER peptide (ab39817).
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Liver X nuclear receptor beta antibody
    • Liver X receptor beta antibody
    • LX receptor beta antibody
    • LXR b antibody
    • LXR beta antibody
    • LXRB antibody
    • NER antibody
    • NER I antibody
    • NER1 antibody
    • NR1H 2 antibody
    • NR1H2 antibody
    • NR1H2_HUMAN antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor NER antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • OR-1 antibody
    • Oxysterols receptor LXR-beta antibody
    • RIP15 antibody
    • Steroid hormone nuclear receptor NER antibody
    • Ubiquitously-expressed nuclear receptor antibody
    • UNR antibody
    see all

Images

  • All lanes : Anti-LXR beta/NER antibody (ab28479) at 1/1000 dilution

    Lane 1 : Rat liver cell lysate
    Lane 2 : Mouse liver cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit

    Predicted band size: 51 kDa

  • ab28479 labelling LXR beta/NER (green) in the nuclear envelope of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab28479 staining LXR beta in C2C12 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue and F-actin stained red. Panel d shows the merged image

References

This product has been referenced in:
  • Mukwaya A  et al. Time-dependent LXR/RXR pathway modulation characterizes capillary remodeling in inflammatory corneal neovascularization. Angiogenesis 21:395-413 (2018). WB, IHC-P ; Rat . Read more (PubMed: 29445990) »
  • Pan LL  et al. (-)-Epigallocatechin-3-Gallate Ameliorates Atherosclerosis and Modulates Hepatic Lipid Metabolic Gene Expression in Apolipoprotein E Knockout Mice: Involvement of TTC39B. Front Pharmacol 9:195 (2018). Read more (PubMed: 29593532) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - 0.3% triton-X
Specification
brain
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Margarita Curras-Collazo

Verified customer

Submitted Oct 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up