LY 294002, PI3-kinase inhibitor (ab120243)

Submit a review Q&A (1)References (31)
Chemical Structure - LY 294002, PI3-kinase inhibitor (ab120243)
  • Western blot - LY 294002, PI3-kinase inhibitor (ab120243)

Key features and details

  • PI3-kinase inhibitor
  • CAS Number: 154447-36-6
  • Purity: > 99%
  • Soluble in DMSO to 25 mM and in ethanol to 5 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    LY 294002, PI3-kinase inhibitor

  • Description

    PI3-kinase inhibitor

  • Biological description

    A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).

  • Purity

    > 99%

  • CAS Number

    154447-36-6

  • Chemical structure

    Chemical Structure

Properties

Images

  • Chemical Structure - LY 294002, PI3-kinase inhibitor (ab120243)
    Chemical Structure - LY 294002, PI3-kinase inhibitor (ab120243)
    2D chemical structure image of ab120243, LY 294002, PI3-kinase inhibitor
  • Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
    Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
    Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

    Lane 1: Marker
    Lane 2: A431 lysate starved
    Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
    Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
    Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

    Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

    Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

    Observed bands:
    RSK1p90 (pS380) band size: 83 kDa
    AKT1 (pS473) band size: 56 kDa
    ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
    RPS6 (pS235/236) band size: 31 kDa
    Rab11a band size: 24 kDa
    Unknown band: 130kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (31)

Publishing research using ab120243? Please let us know so that we can cite the reference in this datasheet.

ab120243 has been referenced in 31 publications.

  • Guan Y & Wang X Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway. Med Sci Monit 27:e928153 (2021). PubMed: 33770068
  • Tanaka Y  et al. NECTIN4: A Novel Therapeutic Target for Melanoma. Int J Mol Sci 22:N/A (2021). PubMed: 33478111
  • Chen L  et al. The Protective Effects of Shengmai Formula Against Myocardial Injury Induced by Ultrafine Particulate Matter Exposure and Myocardial Ischemia are Mediated by the PI3K/AKT/p38 MAPK/Nrf2 Pathway. Front Pharmacol 12:619311 (2021). PubMed: 33762941
  • Liu P  et al. The NRF2-LOC344887 signaling axis suppresses pulmonary fibrosis. Redox Biol 38:101766 (2021). PubMed: 33126057
  • Zhu Y  et al. WISP1 indicates poor prognosis and regulates cell proliferation and apoptosis in gastric cancer via targeting AKT/mTOR signaling pathway. Am J Transl Res 12:7297-7311 (2020). PubMed: 33312368
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Dear technical team,

Our customer purchased ab120243 (LY294002) who used *competitor* LY294002.

The customer thought they were the same but, inhibition rate were quite different.

So, he had complaint about that but, I found out their Cas# are different.

Please let me know the differences between them.

Thank you in advance.


Best regards,

Read More
Answer

Thank you for your inquiry. I am sorry to hear ab120243 is not providing satisfying results.

Could you please let me know how and in what the customer dissolved ab120243?

I can confirm that the CAS number of the *competitor* product and ab120243 is not the same. The *competitor* product is the hydrochloride (HCL)of LY 294002. Please review the formula: C19H17NO3 · HCl.

It also not as pure as ab120243.

Could your customer please provide more details about the experiment, the dilution and the results? This will enable us to better investigate this case for your customer.

I am looking forward to your answer and the requested details.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com