LY 294002, PI3-kinase inhibitor (ab120243)
Key features and details
- PI3-kinase inhibitor
- CAS Number: 154447-36-6
- Soluble in DMSO to 25 mM and in ethanol to 5 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
LY 294002, PI3-kinase inhibitor -
Description
PI3-kinase inhibitor -
Biological description
A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).
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CAS Number
154447-36-6 -
Chemical structure
Properties
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Chemical name
2-(4-Morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one -
Molecular weight
307.35 -
Molecular formula
C19H17NO3 -
PubChem identifier
3973 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM and in ethanol to 5 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C2C=C(Oc1c(cccc12)c3ccccc3)N4CCOCC4 -
Source
Synthetic
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Research areas
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
Images
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Chemical Structure - LY 294002, PI3-kinase inhibitor (ab120243)2D chemical structure image of ab120243, LY 294002, PI3-kinase inhibitor
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Western blot - LY 294002, PI3-kinase inhibitor (ab120243)Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.
Lane 1: Marker
Lane 2: A431 lysate starved
Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)
Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution
Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.
Observed bands:
RSK1p90 (pS380) band size: 83 kDa
AKT1 (pS473) band size: 56 kDa
ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
RPS6 (pS235/236) band size: 31 kDa
Rab11a band size: 24 kDa
Unknown band: 130kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (31)
ab120243 has been referenced in 31 publications.
- Guan Y & Wang X Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway. Med Sci Monit 27:e928153 (2021). PubMed: 33770068
- Tanaka Y et al. NECTIN4: A Novel Therapeutic Target for Melanoma. Int J Mol Sci 22:N/A (2021). PubMed: 33478111
- Chen L et al. The Protective Effects of Shengmai Formula Against Myocardial Injury Induced by Ultrafine Particulate Matter Exposure and Myocardial Ischemia are Mediated by the PI3K/AKT/p38 MAPK/Nrf2 Pathway. Front Pharmacol 12:619311 (2021). PubMed: 33762941
- Liu P et al. The NRF2-LOC344887 signaling axis suppresses pulmonary fibrosis. Redox Biol 38:101766 (2021). PubMed: 33126057
- Zhu Y et al. WISP1 indicates poor prognosis and regulates cell proliferation and apoptosis in gastric cancer via targeting AKT/mTOR signaling pathway. Am J Transl Res 12:7297-7311 (2020). PubMed: 33312368
