LY 294002, PI3-kinase inhibitor (ab120243)

Submit a review Q&A (1)References (24)
Western blot - LY 294002, PI3-kinase inhibitor (ab120243)

    Key features and details

    • PI3-kinase inhibitor
    • CAS Number: 154447-36-6
    • Purity: > 99%
    • Soluble in DMSO to 25 mM and in ethanol to 5 mM
    • Form / State: Solid
    • Source: Synthetic

    Overview

    • Product name

      LY 294002, PI3-kinase inhibitor

    • Description

      PI3-kinase inhibitor

    • Biological description

      A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).

    • Purity

      > 99%

    • CAS Number

      154447-36-6

    • Chemical structure

      Chemical Structure

    Properties

    Associated products

      Images

      • Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
        Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
        Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

        Lane 1: Marker
        Lane 2: A431 lysate starved
        Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
        Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
        Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

        Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

        Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

        Observed bands:
        RSK1p90 (pS380) band size: 83 kDa
        AKT1 (pS473) band size: 56 kDa
        ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
        RPS6 (pS235/236) band size: 31 kDa
        Rab11a band size: 24 kDa
        Unknown band: 130kDa

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (24)

      Publishing research using ab120243? Please let us know so that we can cite the reference in this datasheet.

      ab120243 has been referenced in 24 publications.

      • Feng C  et al. Neuroprotective Effect of Danhong Injection on Cerebral Ischemia-Reperfusion Injury in Rats by Activation of the PI3K-Akt Pathway. Front Pharmacol 11:298 (2020). PubMed: 32218735
      • Zhao M  et al. Vinpocetine Protects Against Cerebral Ischemia-Reperfusion Injury by Targeting Astrocytic Connexin43 via the PI3K/AKT Signaling Pathway. Front Neurosci 14:223 (2020). PubMed: 32300287
      • Zhang J  et al. SNCG promotes the progression and metastasis of high-grade serous ovarian cancer via targeting the PI3K/AKT signaling pathway. J Exp Clin Cancer Res 39:79 (2020). PubMed: 32381034
      • Chen S  et al. WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m6A-dependent manner. Cell Death Dis 11:659 (2020). PubMed: 32814762
      • Yan W  et al. Suppression of SEMA6C promotes preantral follicles atresia with decreased cell junctions in mice ovaries. J Cell Physiol 234:4934-4943 (2019). PubMed: 30256425
      View all Publications for this product

      Customer reviews and Q&As

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      Question

      Dear technical team,

      Our customer purchased ab120243 (LY294002) who used *competitor* LY294002.

      The customer thought they were the same but, inhibition rate were quite different.

      So, he had complaint about that but, I found out their Cas# are different.

      Please let me know the differences between them.

      Thank you in advance.


      Best regards,

      Read More
      Answer

      Thank you for your inquiry. I am sorry to hear ab120243 is not providing satisfying results.

      Could you please let me know how and in what the customer dissolved ab120243?

      I can confirm that the CAS number of the *competitor* product and ab120243 is not the same. The *competitor* product is the hydrochloride (HCL)of LY 294002. Please review the formula: C19H17NO3 · HCl.

      It also not as pure as ab120243.

      Could your customer please provide more details about the experiment, the dilution and the results? This will enable us to better investigate this case for your customer.

      I am looking forward to your answer and the requested details.

      Read More

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