LY 294002, PI3-kinase inhibitor (ab120243)

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Overview

  • Product name
    LY 294002, PI3-kinase inhibitor
  • Description
    PI3-kinase inhibitor
  • Biological description
    A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).
  • Purity
    > 99%
  • CAS Number
    154447-36-6
  • Chemical structure
    Chemical Structure

Properties

Images

  • Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
    Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
    Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

    Lane 1: Marker
    Lane 2: A431 lysate starved
    Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
    Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
    Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

    Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

    Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

    Observed bands:
    RSK1p90 (pS380) band size: 83 kDa
    AKT1 (pS473) band size: 56 kDa
    ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
    RPS6 (pS235/236) band size: 31 kDa
    Rab11a band size: 24 kDa
    Unknown band: 130kDa

References

This product has been referenced in:
  • Gharbi SI  et al. Exploring the specificity of the PI3K family inhibitor LY294002. Biochem J 404:15-21 (2007). Read more (PubMed: 17302559) »
  • Walker EH  et al. Structural determinants of phosphoinositide 3-kinase inhibition by wortmannin, LY294002, quercetin, myricetin, and staurosporine. Mol Cell 6:909-19 (2000). Read more (PubMed: 11090628) »
  • Vlahos CJ  et al. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J Biol Chem 269:5241-8 (1994). Read more (PubMed: 8106507) »
See all 10 Publications for this product

Publishing research using ab120243? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Dear technical team,

Our customer purchased ab120243 (LY294002) who used *competitor* LY294002.

The customer thought they were the same but, inhibition rate were quite different.

So, he had complaint about that but, I found out their Cas# are different.

Please let me know the differences between them.

Thank you in advance.


Best regards,

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Answer

Thank you for your inquiry. I am sorry to hear ab120243 is not providing satisfying results.

Could you please let me know how and in what the customer dissolved ab120243?

I can confirm that the CAS number of the *competitor* product and ab120243 is not the same. The *competitor* product is the hydrochloride (HCL)of LY 294002. Please review the formula: C19H17NO3 · HCl.

It also not as pure as ab120243.

Could your customer please provide more details about the experiment, the dilution and the results? This will enable us to better investigate this case for your customer.

I am looking forward to your answer and the requested details.

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