Recombinant Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38] - BSA and Azide free (ab255803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22502-38] to Ly-6A/E (Sca-1) - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38] - BSA and Azide free
See all Ly-6A/E (Sca-1) primary antibodies -
Description
Rabbit monoclonal [EPR22502-38] to Ly-6A/E (Sca-1) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Concanavalin A treated mouse splenocytes. Mouse bone marrow cells.
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General notes
ab255803 is the carrier-free version of ab219794.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22502-38 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
T-cell activation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 UPAR/Ly6 domain. -
Post-translational
modificationsO-glycosylated. Not N-glycosylated.
Not phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
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Alternative names
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
see all
Images
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Flow cytometric analysis of mouse bone marrow cells labeling Ly-6A/E (Sca1) with ab219794 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219794).
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Flow cytometric analysis of mouse splenocytes treated with 2.5μg/ml Concanavalin A (ConA) for 72h labeling Ly-6A/E (Sca1) with ab219794 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219794).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab255803 has not yet been referenced specifically in any publications.