Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22502-38] to Ly-6A/E - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
Product nameAnti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free
See all Ly-6A/E primary antibodies
DescriptionRabbit monoclonal [EPR22502-38] to Ly-6A/E - BSA and Azide free
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse Ly-6A/E aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P05533
- Flow Cyt: Concanavalin A treated mouse splenocytes. Mouse bone marrow cells.
Ab255803 is the carrier-free version of ab219794. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab255803 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab255803 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
RelevanceLy6A/E is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. Ly6A/E may be involved in B and T lymphocyte responses and it appears that the anitgen may be required for T cell receptor mediated T cell activation.
Cellular localizationCell membrane, attached to the membrane by a GPI anchor.
- Ly 6E antibody
- Ly6A.2 antibody
- Ly6A/E antibody
Flow cytometric analysis of mouse bone marrow cells labeling Ly-6A/E with ab219794 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219794).
Flow cytometric analysis of mouse splenocytes treated with 2.5μg/ml Concanavalin A (ConA) for 72h labeling Ly-6A/E with ab219794 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219794).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab255803 has not yet been referenced specifically in any publications.