Key features and details
- Rabbit polyclonal to Ly6c
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Ly6c antibody
See all Ly6c primary antibodies
DescriptionRabbit polyclonal to Ly6c
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Ly6c aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse thymus tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab77766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa).|
RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
Anti-Ly6c antibody (ab77766) at 1 µg/ml + Thymus (Mouse) Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
We hypothesize that the 11 kDa represents the mature form of Mouse Lymphocyte antigen 6C precursor. Ly6c has a 26 amino acid signal peptide which, when cleaved, gives an expected molecular weight of 11 kDa.
ab77766 has been referenced in 1 publication.
- MohanKumar K et al. A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions. Nat Commun 10:3494 (2019). PubMed: 31375667