Anti-Ly6c antibody (ab77766)
Key features and details
- Rabbit polyclonal to Ly6c
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Ly6c antibody
See all Ly6c primary antibodies -
Description
Rabbit polyclonal to Ly6c -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse Ly6c aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab96115) -
Positive control
- This antibody gave a positive signal in Mouse thymus tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa). |
Target
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Relevance
Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor. -
Database links
- Entrez Gene: 17067 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
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Alternative names
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
see all
Images
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Anti-Ly6c antibody (ab77766) at 1 µg/ml + Thymus (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
We hypothesize that the 11 kDa represents the mature form of Mouse Lymphocyte antigen 6C precursor. Ly6c has a 26 amino acid signal peptide which, when cleaved, gives an expected molecular weight of 11 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab77766 has been referenced in 3 publications.
- Shu B et al. The METTL3/MALAT1/PTBP1/USP8/TAK1 axis promotes pyroptosis and M1 polarization of macrophages and contributes to liver fibrosis. Cell Death Discov 7:368 (2021). PubMed: 34839365
- Zhou J et al. HDAC1-mediated deacetylation of LSD1 regulates vascular calcification by promoting autophagy in chronic renal failure. J Cell Mol Med 24:8636-8649 (2020). PubMed: 32596952
- MohanKumar K et al. A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions. Nat Commun 10:3494 (2019). PubMed: 31375667