• Product name
    Anti-Ly6c antibody [ER-MP20] (Biotin)
    See all Ly6c primary antibodies
  • Description
    Rat monoclonal [ER-MP20] to Ly6c (Biotin)
  • Host species
  • Conjugation
  • Specificity
    ab15674 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
    Database link: P0CW02

  • Epitope
    Spleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line.
  • Positive control
    • Monoblasts, late CFU M cells, monocytes, granulocytes. Macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs. Activated macrophages in inflammatory tissues.
  • General notes

    ab15674 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.



Our Abpromise guarantee covers the use of ab15674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.



  • Relevance
    Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all


ab15674 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for contacting us.

I have our storage department looking into this, as the customer should have received the 250 ul. I am also checking with our datasheet department.

In the meanwhile, I apologize for this to happen, and hope to come back with an explanation and a solution shortly.

I hope this information is helpful to you.

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Thank you for email.

Please check with our customer service as I can issue an FOCR, but have nothing to do with the order system. Maybe they can transfer the FOCR to an order which willbe shipped earlier?

Sorry I cannot help youwith thiscase.

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Thank you for your email.

No, the customer is correct, he recieved a shortage instead of the 250ul on the datasheet.

We would be pleased to arrange a replacement product free of charge for this shortage, and this will delivered on Order#123456.

Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab9183. The estimated delivery date will be January 24th, 2012.I hope this will not be a big problem for you. I apologize for any inconvenience.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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