Overview

  • Product name
    Anti-Ly6c antibody [HK1.4]
    See all Ly6c primary antibodies
  • Description
    Rat monoclonal [HK1.4] to Ly6c
  • Host species
    Rat
  • Tested applications
    Suitable for: Cellular Activation, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    L3 cloned CTL cells

Properties

Applications

Our Abpromise guarantee covers the use of ab24973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Cellular Activation
Flow Cyt
IHC-Fr
  • Application notes
    Cellular activation: Use at an assay dependent dilution.
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
    • Cellular localization
      Cell membrane; Lipid-anchor, GPI-anchor.
    • Database links
    • Alternative names
      • Ly 6c antibody
      • Ly6c protein antibody
      • Ly6c1 antibody
      • Ly6c2 antibody
      • Lymphocyte antigen 6 complex antibody
      • Lymphocyte antigen 6 complex locus C antibody
      • Lymphocyte antigen 6C antibody
      • Lymphocyte antigen 6C1 antibody
      • Lymphocyte antigen 6C2 antibody
      • Lymphocyte antigen Ly 6C antibody
      see all

    Images

    • Bone marrow cells from C57BL/6 mice were labeled with ab24973 and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.

    References

    This product has been referenced in:
    • Liyanage SE  et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res 151:160-70 (2016). IHC-P . Read more (PubMed: 27544307) »
    • Zhao C  et al. Nuclear Factor E2-related Factor-2 (Nrf2) Is Required for NLRP3 and AIM2 Inflammasome Activation. J Biol Chem 289:17020-17029 (2014). Flow Cyt . Read more (PubMed: 24798340) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target retrival high ph
    Permeabilization
    No
    Specification
    mouse spleen
    Blocking step
    Peroxidase block as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Frau. Ines Kleiber-Schaaf

    Verified customer

    Submitted May 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up