Anti-Ly6c antibody [HK1.4] (ab24973)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-Ly6c antibody [HK1.4]
See all Ly6c primary antibodies -
DescriptionRat monoclonal [HK1.4] to Ly6c
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Host speciesRat
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Tested applicationsSuitable for: Cellular Activation, Flow Cyt, IHC-Frmore details
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Species reactivityReacts with: Mouse
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Immunogen
L3 cloned CTL cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
100mM borate buffered saline, pH 8.0.
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Concentration information loading... -
PurityAffinity purified
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ClonalityMonoclonal
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Clone numberHK1.4
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IsotypeIgG2c
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab24973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Cellular Activation | ||
| Flow Cyt | ||
| IHC-Fr |
Application notes
Cellular activation: Use at an assay dependent dilution.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
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Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
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Database links
- Entrez Gene: 17067 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
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Alternative names
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
see all
Images
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![Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)](https://www.abcam.com/ps/products/24/ab24973/Images/ab24973_1.jpg)
Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)Bone marrow cells from C57BL/6 mice were labeled with ab24973 and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.
Datasheets and documents
References
This product has been referenced in:
- Liyanage SE et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res 151:160-70 (2016). IHC-P . Read more (PubMed: 27544307) »
- Zhao C et al. Nuclear Factor E2-related Factor-2 (Nrf2) Is Required for NLRP3 and AIM2 Inflammasome Activation. J Biol Chem 289:17020-17029 (2014). Flow Cyt . Read more (PubMed: 24798340) »
