Anti-Ly6c antibody [HK1.4] (ab24973)
Key features and details
- Rat monoclonal [HK1.4] to Ly6c
- Suitable for: Cellular Activation, Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2c
Overview
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Product name
Anti-Ly6c antibody [HK1.4]
See all Ly6c primary antibodies -
Description
Rat monoclonal [HK1.4] to Ly6c -
Host species
Rat -
Tested applications
Suitable for: Cellular Activation, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
L3 cloned CTL cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: None
100mM borate buffered saline, pH 8.0.
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Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
HK1.4 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab24973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Cellular Activation | ||
| Flow Cyt | ||
| IHC-Fr |
Application notes
Cellular activation: Use at an assay dependent dilution.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor. -
Database links
- Entrez Gene: 17067 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
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Alternative names
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
see all
Images
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![Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)](https://www.abcam.com/ps/products/24/ab24973/Images/ab24973-35748-anti-ly6c-antibody-hk14-flow-cytometry.jpg)
Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)Bone marrow cells from C57BL/6 mice were labeled with ab24973 and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.
Protocols
Datasheets and documents
References
ab24973 has been referenced in 5 publications.
- Feng Z et al. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death Dis 8:e3170 (2017). PubMed: 29144508
- Liyanage SE et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res 151:160-70 (2016). IHC-P . PubMed: 27544307
- Zhao C et al. Nuclear Factor E2-related Factor-2 (Nrf2) Is Required for NLRP3 and AIM2 Inflammasome Activation. J Biol Chem 289:17020-17029 (2014). Flow Cyt . PubMed: 24798340
- Scindia Y et al. Activated CD4+ T cells target mesangial antigens and initiate glomerulonephritis. Nephron Exp Nephrol 121:e1-9 (2012). PubMed: 23095207
- Makaroff LE et al. Postthymic maturation influences the CD8 T cell response to antigen. Proc Natl Acad Sci U S A 106:4799-804 (2009). Flow Cyt ; Mouse . PubMed: 19270077
