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  1. Link

    ly6c-antibody-hk14-ab24973.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Ly6c antibody [HK1.4] (ab24973)

  • Datasheet
Reviews (2) Submit a question References (5)

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Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)

    Key features and details

    • Rat monoclonal [HK1.4] to Ly6c
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2c

    Conjugates logo Related conjugates and formulations

    APC Biotin FITC PE

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    Overview

    • Product name

      Anti-Ly6c antibody [HK1.4]
      See all Ly6c primary antibodies
    • Description

      Rat monoclonal [HK1.4] to Ly6c
    • Host species

      Rat
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Ly6c. L3 cloned CTL cells

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 8.2
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      HK1.4
    • Isotype

      IgG2c
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD
      • Cell Biology
      • Cell Cycle
      • Cell differentiation
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage

    Associated products

    • Alternative Versions

      • Biotin Anti-Ly6c antibody [HK1.4] (ab24980)
      • FITC Anti-Ly6c antibody [HK1.4] (ab25025)
      • PE Anti-Ly6c antibody [HK1.4] (ab25572)
      • APC Anti-Ly6c antibody [HK1.4] (ab93550)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Recombinant Protein

      • Recombinant Mouse Ly6c protein (Tagged) (ab240852)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab24973 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Notes
    Application notes
    Cellular activation: Use at an assay dependent dilution.
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Relevance

      Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
    • Cellular localization

      Cell membrane; Lipid-anchor, GPI-anchor.
    • Database links

      • Entrez Gene: 17067 Mouse
      • SwissProt: P0CW02 Mouse
      • SwissProt: P0CW03 Mouse
      • Alternative names

        • Ly 6c antibody
        • Ly6c protein antibody
        • Ly6c1 antibody
        • Ly6c2 antibody
        • Lymphocyte antigen 6 complex antibody
        • Lymphocyte antigen 6 complex locus C antibody
        • Lymphocyte antigen 6C antibody
        • Lymphocyte antigen 6C1 antibody
        • Lymphocyte antigen 6C2 antibody
        • Lymphocyte antigen Ly 6C antibody
        see all

      Images

      • Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)
        Flow Cytometry - Anti-Ly6c antibody [HK1.4] (ab24973)

        Bone marrow cells from C57BL/6 mice were labeled with ab24973 and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab24973? Please let us know so that we can cite the reference in this datasheet.

      ab24973 has been referenced in 5 publications.

      • Feng Z  et al. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death Dis 8:e3170 (2017). PubMed: 29144508
      • Liyanage SE  et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res 151:160-70 (2016). IHC-P . PubMed: 27544307
      • Zhao C  et al. Nuclear Factor E2-related Factor-2 (Nrf2) Is Required for NLRP3 and AIM2 Inflammasome Activation. J Biol Chem 289:17020-17029 (2014). Flow Cyt . PubMed: 24798340
      • Scindia Y  et al. Activated CD4+ T cells target mesangial antigens and initiate glomerulonephritis. Nephron Exp Nephrol 121:e1-9 (2012). PubMed: 23095207
      • Makaroff LE  et al. Postthymic maturation influences the CD8 T cell response to antigen. Proc Natl Acad Sci U S A 106:4799-804 (2009). Flow Cyt ; Mouse . PubMed: 19270077

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Ly6c antibody [HK1.4]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Mouse Liver Kupffer Cells)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Mouse Liver Kupffer Cells
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      DR. Armen Petrosyan

      Verified customer

      Submitted Oct 13 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ly6c antibody [HK1.4]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (mouse spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Target retrival high ph
      Permeabilization
      No
      Specification
      mouse spleen
      Blocking step
      Peroxidase block as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      FRAU. Ines Kleiber-Schaaf

      Verified customer

      Submitted May 03 2018

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