Key features and details
- Rat monoclonal [HK1.4] to Ly6c (PE)
- Suitable for: Flow Cyt, IHC-Fr, Cellular Activation
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2c
Product nameAnti-Ly6c antibody [HK1.4] (PE)
See all Ly6c primary antibodies
DescriptionRat monoclonal [HK1.4] to Ly6c (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab25572 recognises Ly6c.
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Cellular Activationmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent.
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab25572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.
ab154450 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration. In vitro activation of T lymphocytes.|
RelevanceLy6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
Flow cytometry analysis of murine splenocytes, staining Ly6c with ab25572.
Cells were cultured in Cells were isolated via mechanical disruption of the spleen. The sample was incubated with the primary antibody (1/100 in 2% FCS + 0.5 mM EDTA in PBS) for 30 minutes at 4°C.
Gating Strategy: lymphocytes
ab25572 has been referenced in 2 publications.
- Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234
- Ocuin LM et al. Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. J Leukoc Biol 89:423-32 (2011). Flow Cyt ; Mouse . PubMed: 21106642