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  1. Link

    ly6g-antibody-1a8-low-endotoxin-azide-free-ab210204.pdf

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Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)

  • Datasheet
Submit a review Submit a question References (8)

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Abpromise

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Find out more.

Key features and details

  • Rat monoclonal [1A8] to Ly6g - Low endotoxin, Azide free
  • Suitable for: IHC-Fr, IHC-P, IP, Flow Cyt, Functional Studies
  • Reacts with: Mouse
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

APC FITC PE/Cy7® PerCP/Cy5.5® redFluor™ 710 violetFluor™ 450

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Protein
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Recombinant Mouse Ly6g protein (His tag) (ab241265)

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Overview

  • Product name

    Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free
    See all Ly6g primary antibodies
  • Description

    Rat monoclonal [1A8] to Ly6g - Low endotoxin, Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, IP, Flow Cyt, Functional Studiesmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Endotoxin Level: Less than or equal to 0.01 EU/μg, as determined by the LaL assay.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituents: 0.87% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab210204 was purified from tissue culture supernatant via affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    1A8
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • APC Anti-Ly6g antibody [1A8] (ab210201)
    • FITC Anti-Ly6g antibody [1A8] (ab210203)
    • PE/Cy7® Anti-Ly6g antibody [1A8] (ab210206)
    • PerCP/Cy5.5® Anti-Ly6g antibody [1A8] (ab210207)
    • redFluor™ 710 Anti-Ly6g antibody [1A8] (ab242066)
    • violetFluor™ 450 Anti-Ly6g antibody [1A8] (ab242074)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant Mouse Ly6g protein (His tag) (ab241265)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.

Target

  • Relevance

    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • Cellular localization

    Attached to the membrane by a GPI-anchor.
  • Database links

    • Entrez Gene: 546644 Mouse
    • SwissProt: P35461 Mouse
    • Alternative names

      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (8)

    Publishing research using ab210204? Please let us know so that we can cite the reference in this datasheet.

    ab210204 has been referenced in 8 publications.

    • Dhanesha N  et al. Cellular fibronectin promotes deep vein thrombosis in diet-induced obese mice. J Thromb Haemost 19:814-821 (2021). PubMed: 33300307
    • Tran A  et al. Estrogen-related receptor alpha (ERRa) is a key regulator of intestinal homeostasis and protects against colitis. Sci Rep 11:15073 (2021). PubMed: 34302001
    • Liang Y  et al. Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model. Zool Res 41:621-631 (2020). PubMed: 33045777
    • Zhou J  et al. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment. J Leukoc Biol 105:177-186 (2019). PubMed: 30351498
    • Winstel V  et al. Staphylococcus aureus Exploits the Host Apoptotic Pathway To Persist during Infection. mBio 10:N/A (2019). PubMed: 31719177
    • Wen G  et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
    • Verbiest T  et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia 32:1435-1444 (2018). PubMed: 29556020
    • Feng Z  et al. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death Dis 8:e3170 (2017). PubMed: 29144508

    Customer reviews and Q&As

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