Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)
Key features and details
- Rat monoclonal [1A8] to Ly6g - Low endotoxin, Azide free
- Suitable for: IP, Flow Cyt, Functional Studies, IHC-Fr, IHC-P
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free
See all Ly6g primary antibodies -
Description
Rat monoclonal [1A8] to Ly6g - Low endotoxin, Azide free -
Host species
Rat -
Tested applications
Suitable for: IP, Flow Cyt, Functional Studies, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
Endotoxin Level: Less than or equal to 0.01 EU/μg, as determined by the LaL assay.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.87% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab210204 was purified from tissue culture supernatant via affinity chromatography. -
Clonality
Monoclonal -
Clone number
1A8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Anti-Ly6g antibody [1A8] (APC) (ab210201)
- Anti-Ly6g antibody [1A8] (FITC) (ab210203)
- Anti-Ly6g antibody [1A8] (PE/Cy7®) (ab210206)
- Anti-Ly6g antibody [1A8] (PerCP/Cy5.5®) (ab210207)
- Anti-Ly6g antibody [1A8] (CF405M) (ab236484)
- Anti-Ly6g antibody [1A8] (PerCP) (ab236485)
- Anti-Ly6g antibody [1A8] (redFluor™ 710) (ab242066)
- Anti-Ly6g antibody [1A8] (violetFluor™ 450) (ab242074)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab210204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Relevance
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. -
Cellular localization
Attached to the membrane by a GPI-anchor. -
Database links
- Entrez Gene: 546644 Mouse
- SwissProt: P35461 Mouse
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Alternative names
- Gr-1 antibody
- Gr1 antibody
- Ly-6G.1 antibody
see all
Protocols
Datasheets and documents
References
ab210204 has been referenced in 4 publications.
- Zhou J et al. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment. J Leukoc Biol 105:177-186 (2019). PubMed: 30351498
- Winstel V et al. Staphylococcus aureus Exploits the Host Apoptotic Pathway To Persist during Infection. mBio 10:N/A (2019). PubMed: 31719177
- Wen G et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
- Feng Z et al. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death Dis 8:e3170 (2017). PubMed: 29144508
