Recombinant
RabMAb

Recombinant Anti-Ly6g antibody [EPR22909-135] (ab238132)

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Overview

  • Product name

    Anti-Ly6g antibody [EPR22909-135]
    See all Ly6g primary antibodies

  • Description

    Rabbit monoclonal [EPR22909-135] to Ly6g

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse Ly6g aa 1-100. The exact sequence is proprietary.
    Database link: P35461

  • Positive control

    • IHC-P: Mouse spleen and lung tissues. FC: Mouse peripheral blood mononuclear cell and HEK-293T cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/600.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF,IP or WB.

    • Target

    • Relevance

      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

    • Cellular localization

      Attached to the membrane by a GPI-anchor.

    • Database links

      • Alternative names

        • Gr-1 antibody
        • Gr1 antibody
        • Ly-6G.1 antibody
        • Ly6G antibody
        • Lymphocyte antigen 6 complex locus G antibody
        • Lymphocyte antigen 6G antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in mouse spleen (PMID: 21976773) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in mouse lung (PMID: 22434870) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Cells were stained with isotype control rabbit monoclonal IgG ab172730 (Left) or ab238132 (Right), then stained with anti-CD11b conjugated to BV421.
        Gated on viable cells.

      • Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with HA-tagged Ly6c overexpression construct (Left) / HEK-293T transfected with HA-tagged Ly6g overexpression construct (Right) cells, labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Ly6c (Left) and Ly6g (Right) overexpression cells were surface stained with ab238132 and anti-HA tag conjugated to Alexa Fluor®647.

        ab238132 does not cross-react with Ly6c.

        Gated on viable cells.

      References

      ab238132 has not yet been referenced specifically in any publications.

      Publishing research using ab238132? Please let us know so that we can cite the reference in this datasheet.

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