RecombinantRabMAb

Recombinant Anti-Ly6g antibody [EPR22909-135] (ab238132)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
  • Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
  • Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
  • Anti-Ly6g antibody [EPR22909-135] (ab238132)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22909-135] to Ly6g
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Ly6g antibody [EPR22909-135]
    See all Ly6g primary antibodies

  • Description

    Rabbit monoclonal [EPR22909-135] to Ly6g

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and lung tissues. Flow Cyt: Mouse peripheral blood mononuclear cell and HEK-293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Mouse
Human
ICC
Mouse
IHC-P
Mouse
IP
Mouse
WB
Mouse
Rat
Human
Application Abreviews Notes
Flow Cyt
1/600.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt
1/600.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF,IP or WB.

    • Target

    • Relevance

      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

    • Cellular localization

      Attached to the membrane by a GPI-anchor.

    • Database links

      • Alternative names

        • Gr-1 antibody
        • Gr1 antibody
        • Ly-6G.1 antibody
        • Ly6G antibody
        • Lymphocyte antigen 6 complex locus G antibody
        • Lymphocyte antigen 6G antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in mouse spleen (PMID: 21976773) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in mouse lung (PMID: 22434870) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Cells were stained with isotype control rabbit monoclonal IgG ab172730 (Left) or ab238132 (Right), then stained with anti-CD11b conjugated to BV421.
        Gated on viable cells.

      • Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)

        Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with HA-tagged Ly6c overexpression construct (Left) / HEK-293T transfected with HA-tagged Ly6g overexpression construct (Right) cells, labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Ly6c (Left) and Ly6g (Right) overexpression cells were surface stained with ab238132 and anti-HA tag conjugated to Alexa Fluor®647.

        ab238132 does not cross-react with Ly6c.

        Gated on viable cells.

      • Anti-Ly6g antibody [EPR22909-135] (ab238132)
        Anti-Ly6g antibody [EPR22909-135] (ab238132)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab238132? Please let us know so that we can cite the reference in this datasheet.

      ab238132 has not yet been referenced specifically in any publications.

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