Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377)
Key features and details
- Rat monoclonal [RB6-8C5] to Ly6g + Ly6c
- Suitable for: IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Ly6g + Ly6c antibody [RB6-8C5] -
Description
Rat monoclonal [RB6-8C5] to Ly6g + Ly6c -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
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General notes
This product was changed from ascites to tissue culture supernatant on 2nd March 2020. Lot numbers higher than GR3218400 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
RB6-8C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (ab242065)
- PE Anti-Ly6g antibody [RB6-8C5] (ab24884)
- FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
- APC Anti-Ly6g antibody [RB6-8C5] (ab25273)
- violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
- PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (ab25514)
- Biotin Anti-Ly6g antibody [RB6-8C5] (ab25680)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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IHC-P | (4) |
Use at an assay dependent concentration. PubMed: 25126943
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 25126943 |
Target
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Cellular localization
Ly6g: Attached to the membrane by a GPI-anchor. Ly6c: Cell membrane; Lipid-anchor, GPI-anchor. -
Database links
- Entrez Gene: 17067 Mouse
- Entrez Gene: 546644 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
- SwissProt: P35461 Mouse
Images
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Immunohistochemistry (Frozen sections) analysis of Mouse frozen spleen tissue labeling Ly6g with ab25377. Right: Rat Anti-Mouse Ly-6G-UNLB followed by Mouse Anti-Rat IgG2b-FITC. Left: Isotype Control, Rat IgG2b-UNLB followed by Mouse Anti-Rat IgG2b-FITC. Blue - DAPI.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (172)
ab25377 has been referenced in 172 publications.
- Missiroli S et al. PML at mitochondria-associated membranes governs a trimeric complex with NLRP3 and P2X7R that modulates the tumor immune microenvironment. Cell Death Differ 30:429-441 (2023). PubMed: 36450825
- Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol 52:102288 (2022). PubMed: 35325804
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Ngamsri KC et al. Sevoflurane Dampens Acute Pulmonary Inflammation via the Adenosine Receptor A2B and Heme Oxygenase-1. Cells 11:N/A (2022). PubMed: 35406657
- Zhang X & Dong S Protective effect of growth differentiation factor 15 in sepsis by regulating macrophage polarization and its mechanism. Bioengineered 13:9687-9707 (2022). PubMed: 35420978