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Rabbit polyclonal to LY6G5C
antigen from Human LY6G5C
- Human tonsil tissue. RT-4 and U-251 MG lysate. Human Plasma.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May have a role in hematopoietic cell differentiation.
Detected in T-cell lines and fetal and adult lung.
Contains 1 UPAR/Ly6 domain.
Information by UniProt
- C6orf20 antibody
- G5C antibody
- LY65C_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-LY6G5C antibody (ab122501)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122501 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-LY6G5C antibody (ab122501)
All lanes : Anti-LY6G5C antibody (ab122501) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6G5C antibody (ab122501)
Immunohistochemical staining of human tonsil using 1/100 ab122501.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"