Key features and details
- Rabbit polyclonal to LY75/DEC-205
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LY75/DEC-205 antibody
See all LY75/DEC-205 primary antibodies
DescriptionRabbit polyclonal to LY75/DEC-205
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human LY75/DEC-205 aa 440-680.
TEVTLTYWDENEPNVPYNKTPNCVSYLGELGQWKVQSCEEKLKYVCKRKG EKLNDASSDKMCPPDEGWKRHGETCYKIYEDEVPFGTNCNLTITSRFEQE YLNDLMKKYDKSLRKYFWTGLRDVDSCGEYNWATVGGRRRAVTFSNWNFL EPASPGGCVAMSTGKSVGKWEVKDCRSFKALSICKKMSGPLGPEEASPKP DDPCPEGWQSFPASLSCYKVFHAERIVRKRNWEEAERFCQA
Database link: O60449
- WB: Mouse liver tissue lysate. IHC-P: Human tonsil and spleen tissue.
Previously labelled as LY75.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 198 kDa (predicted molecular weight: 216, 210, 198, 20 kDa).|
FunctionActs as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
Tissue specificityExpressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
Sequence similaritiesContains 10 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
- Information by UniProt
- C-type lectin domain family 13 member B antibody
- CD 205 antibody
- CD205 antibody
Anti-LY75/DEC-205 antibody (ab230251) at 1/1000 dilution + Mouse liver tissue lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 216, 210, 198, 20 kDa
Observed band size: 198 kDa why is the actual band size different from the predicted?
Paraffin-embedded human tonsil tissue stained for LY75/DEC-205 with ab230251 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human spleen tissue stained for LY75/DEC-205 with ab230251 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230251 has not yet been referenced specifically in any publications.