Recombinant Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5233] to LY75/DEC-205
- Suitable for: WB, IHC-P, mIHC
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-LY75/DEC-205 antibody [EPR5233]
See all LY75/DEC-205 primary antibodies -
Description
Rabbit monoclonal [EPR5233] to LY75/DEC-205 -
Host species
Rabbit -
Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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Tested applications
Suitable for: WB, IHC-P, mIHCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human LY75/DEC-205 aa 1700-1800 (intracellular). The exact sequence is proprietary.
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Positive control
- WB: Human tonsil lysate, Daudi Whole cell lysate, Mouse lymph node lysate and Mouse thymus lysate IHC: human T cell lymphoma tissue, human thymus tissue, human spleen tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.54 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5233 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 198 kDa).
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IHC-P | (1) |
1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For unpurified use: 1/250.00000 - 1/500.00000 |
mIHC |
Use at an assay dependent concentration.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 198 kDa). |
IHC-P
1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For unpurified use: 1/250.00000 - 1/500.00000 |
mIHC
Use at an assay dependent concentration. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) |
Target
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Function
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes. -
Tissue specificity
Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. -
Sequence similarities
Contains 10 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 100526664 Human
- Entrez Gene: 4065 Human
- Entrez Gene: 17076 Mouse
- Omim: 604524 Human
- SwissProt: O60449 Human
- SwissProt: Q60767 Mouse
- Unigene: 153563 Human
- Unigene: 2074 Mouse
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Alternative names
- C-type lectin domain family 13 member B antibody
- CD 205 antibody
- CD205 antibody
see all
Images
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All lanes : Anti-LY75/DEC-205 antibody [EPR5233] (ab124897) at 1/1000 dilution (Purified)
Lane 1 : Human tonsil lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : Mouse lymph node lysate
Lane 4 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 198 kDa
Observed band size: 205 kDa why is the actual band size different from the predicted?DEC-205 is a 205 kD integral membrane protein abundant on dendritic cells in lymphoid tissues. We are unsure about the nature of the lower extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human t cell lymphoma tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1:1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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This data was developed using ab208649, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human thymus tissue labeling CD3 with ab16669 at 1/500 dilution, LY75/DEC-205 with ab208649 at 1/15000, and CD68 with ab213363 at 1/500 dilution.
Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 (green; Opal™520) and anti-LY75/DEC-205 (red; Opal™570) on human thymus.
Panel B: anti-CD3 stained on T cells.
Panel C: anti-LY75/DEC-205 stained on thymic cortical epithelium and dendritic cells.
Panel D: anti-CD68 stained on macrophages.
Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of ab213363, ab16669, and ab208649 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.DAPI was used as a nuclear counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymus tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1:1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1:1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124897 has been referenced in 2 publications.
- Sato T et al. Intrapulmonary Delivery of CpG Microparticles Eliminates Lung Tumors. Mol Cancer Ther 14:2198-205 (2015). PubMed: 26206336
- D'Auria KM et al. In vivo physiological and transcriptional profiling reveals host responses to Clostridium difficile toxin A and toxin B. Infect Immun 81:3814-24 (2013). PubMed: 23897615