Recombinant
RabMAb

Recombinant Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free (ab208649)

Overview

  • Product name

    Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free
    See all LY75/DEC-205 primary antibodies
  • Description

    Rabbit monoclonal [EPR5233] to LY75/DEC-205 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide within Human LY75/DEC-205 aa 1700-1800 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Human tonsil tissue; Human thymus, lymph node, tonsil and Mouse thymus lysates.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Previously labelled as LY75. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 205 kDa (predicted molecular weight: 198 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
  • Tissue specificity

    Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
  • Sequence similarities

    Contains 10 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-type lectin domain family 13 member B antibody
    • CD 205 antibody
    • CD205 antibody
    • CD205 antigen antibody
    • CLEC 13B antibody
    • CLEC13B antibody
    • DEC 205 antibody
    • DEC-205 antibody
    • DEC205 antibody
    • gp200 antibody
    • gp200 MR6 antibody
    • gp200-MR6 antibody
    • LY 75 antibody
    • Ly-75 antibody
    • LY75 antibody
    • LY75_HUMAN antibody
    • Lymphocyte antigen 75 antibody
    • Renal Cell Carcinoma (gp200) antibody
    see all

Images

  • ab124897, at 1/250 dilution, staining LY75/DEC-205 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing positive staining in Normal thymus tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing positive staining in Normal spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing negative staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing positive staining in Normal colon lymphocytes tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • ab124897 showing negative staining in Normal lung tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124897).

References

This product has been referenced in:

  • D'Auria KM  et al. In vivo physiological and transcriptional profiling reveals host responses to Clostridium difficile toxin A and toxin B. Infect Immun 81:3814-24 (2013). Read more (PubMed: 23897615) »
See 1 Publication for this product

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