Overview

  • Product name

    Anti-Ly76 antibody [TER-119] (Phycoerythrin)
    See all Ly76 primary antibodies
  • Description

    Rat monoclonal [TER-119] to Ly76 (Phycoerythrin)
  • Host species

    Rat
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation - Mouse fetal liver cells

  • Positive control

    • BALB/c bone marrow cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    Ly76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.
  • Cellular localization

    Plasma membrane
  • Database links

  • Alternative names

    • Ly 76 antibody
    • Lymphocyte antigen 76 antibody
    • TER 119 antibody
    • TER119 antibody

Images

  • Flow cytometry of C57Bl/6 bone marrow cells labeling Ly76 with ab93586 at 0.5µg (solid line) or Rat IgG2b isotype control at 0.5µg (dashed line).

References

This product has been referenced in:

  • Kina T  et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280-7 (2000). Read more (PubMed: 10848813) »
  • Vannucchi AM  et al. Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood 95:2559-68 (2000). Read more (PubMed: 10753835) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (Erythrocytes)
Specification
Erythrocytes
Preparation
Cell harvesting/tissue preparation method: Isolation of purified erythrocytes from whole blood using percoll gradient
Sample buffer: PBS with 5mM EDTA
Fixation
Unfixed
Permeabilization
No
Gating Strategy
live cell population of purified erythrocytes gated on the basis of FSC/SSC. P2 gating for positive cell population was set so that <1% of the negative cell population was PE positive.

Abcam user community

Verified customer

Submitted Mar 25 2011

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