Key features and details
- Rabbit polyclonal to LYAR
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LYAR antibody
See all LYAR primary antibodies
DescriptionRabbit polyclonal to LYAR
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human LYAR aa 1-379.
MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVK CISEDQKYGGKGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQ ISAFDNVPRKKAKFQNWMKNSLKVHNESILDQVWNIFSEASNSEPVNKEQ DQRPLHPVANPHAEISTKVPASKVKDAVEQQGEVKKNKRERKEERQKKRK REKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVPEANGSAGKRSK KKKQRKDSASEEEAHVGAGKRKRRHSEVETDSKKKKMKLPEHPEGGEPED DEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRS EEELLVIFNKKISKNPTFKLLKDKVKLVK
Database link: Q9NX58
- WB: K562, HeLa, SH-SY5Y and PC-3 whole cell lysate. IHC-P: Human testis tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 44 kDa.|
Sequence similaritiesContains 2 C2HC-type zinc fingers.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Cell growth regulating nucleolar protein antibody
- Cell growth-regulating nucleolar protein antibody
- Likely ortholog of mouse Ly1 antibody reactive clone antibody
All lanes : Anti-LYAR antibody (ab237738) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Paraffin-embedded human testis tissue stained for LYAR using ab237738 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LYAR (green) using ab237738 at 1/166 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237738 has not yet been referenced specifically in any publications.