Product nameAnti-LYAR antibody
See all LYAR primary antibodies
DescriptionRabbit polyclonal to LYAR
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human LYAR aa 1-379.
MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVK CISEDQKYGGKGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQ ISAFDNVPRKKAKFQNWMKNSLKVHNESILDQVWNIFSEASNSEPVNKEQ DQRPLHPVANPHAEISTKVPASKVKDAVEQQGEVKKNKRERKEERQKKRK REKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVPEANGSAGKRSK KKKQRKDSASEEEAHVGAGKRKRRHSEVETDSKKKKMKLPEHPEGGEPED DEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRS EEELLVIFNKKISKNPTFKLLKDKVKLVK
Database link: Q9NX58
- WB: K562, HeLa, SH-SY5Y and PC-3 whole cell lysate. IHC-P: Human testis tissue. ICC/IF: HeLa cells.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237738 in the following tested applications.
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 44 kDa.|
Sequence similaritiesContains 2 C2HC-type zinc fingers.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Cell growth regulating nucleolar protein antibody
- Cell growth-regulating nucleolar protein antibody
- Likely ortholog of mouse Ly1 antibody reactive clone antibody
All lanes : Anti-LYAR antibody (ab237738) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Paraffin-embedded human testis tissue stained for LYAR using ab237738 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LYAR (green) using ab237738 at 1/166 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
ab237738 has not yet been referenced specifically in any publications.